FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12365
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT371330.02830570157703No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT138711.217145167812374RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT9817.933683784876668RNA PCR Primer, Index 46 (95% over 23bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG7385.968459361099879RNA PCR Primer, Index 46 (96% over 28bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT3913.1621512333198543RNA PCR Primer, Index 46 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGT3723.0084917104731095No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA2822.280630812778002RNA PCR Primer, Index 46 (96% over 28bp)
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG2201.7792155276991506TruSeq Adapter, Index 4 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTT1321.0675293166194906No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGT1241.0028305701577032No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC1190.9623938536190861No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1120.9057824504650221No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC1110.8976951071572987TruSeq Adapter, Index 18 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT990.8006469874646179No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCT830.6712494945410432Illumina PCR Primer Index 8 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGTCTT610.4933279417711282No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG600.4852405984634048No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAACCTCGTATGCCGT600.4852405984634048No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC550.44480388192478765No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT540.43671653861706433No Hit
CTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC480.3881924787707238No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGTCTTCT390.3154063890012131Illumina PCR Primer Index 8 (95% over 21bp)
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTT390.3154063890012131TruSeq Adapter, Index 4 (96% over 26bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGC380.3073190456934897No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.26688232915487264No Hit
TCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCTGCTTG300.2426202992317024Illumina PCR Primer Index 8 (96% over 26bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTAAGGCG300.2426202992317024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.18600889607763849No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAACCTCGTATGCCGTCTT220.1779215527699151No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCCT220.1779215527699151No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCTGCT200.16174686615446826Illumina PCR Primer Index 8 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGATCTCGTATGCCGTC190.15365952284674486RNA PCR Primer, Index 33 (95% over 21bp)
CTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCTGCTTGA190.15365952284674486Illumina PCR Primer Index 8 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAAATCGTATGCCGT160.1293974929235746No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGG160.1293974929235746No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGTCTT150.1213101496158512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.11322280630812777No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCTG140.11322280630812777RNA PCR Primer, Index 46 (95% over 23bp)
CTTAACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC130.10513546300040437No Hit
TCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGTCTTCTGCTTG130.10513546300040437Illumina PCR Primer Index 8 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC130.10513546300040437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)