FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421642

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421642
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94302
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTC3750.3976585862441942No Hit
CCTTAGAAAGATGAAAATGTGAAAAACAACAGTCATAAAATGGAATCGCT3730.3955377404508918No Hit
GTACATGGGATCTCCCTCCGCCTCATTGCCTATTTTGTCCTCAGGTACAA3200.33933532692837903No Hit
GCCTACATCTTCCTAGTTAAAGCTAGAAAGGGGAATGGAGAGAAGATCCA3010.3191872918920065No Hit
TTTCTAAGGTGTGTCTCTTTTCCAATGAGAAGTCATTTTTGCAAGCCAAA2900.3075226400288435No Hit
GTTATAAACAGCGTGATTTTATATATGACATTCTGGAAGAGGCAGTGTTG2350.24919938071302836No Hit
GTACTAAAAAGGTATAATTTCTTAAAATGAATGCGATTGATCGACTTTTG2220.23541388305656297No Hit
GTATGTGATTTACCCTGAATCATTCTTCAGCAAGTACTAAAAAGGTATAA2060.21844711671014402No Hit
CCTTAGCACCAGGCACCGTGGGTCCTGGATGGGGAACTGCAAGCAGCCTC2030.21526584802019047No Hit
TTTTAGTACTTGCTGAAGAATGATTCAGGGTAAATCACATACTTTGTTTA1820.19299696719051557No Hit
GATTAGACACTGTGGCCGAGGGCACGTCTAGAATCGAGGAGGCAAGCCTG1820.19299696719051557No Hit
CCTTCAGCCCTCACCCCTGGTTCTCATCTTTCTTGATCAACATCTTTTCT1770.18769485270725966No Hit
GTCTAGAATCGAGGAGGCAAGCCTGTGCCCGACCGACGACACAGAGACTC1610.1707280863608407No Hit
GTTCTAGGGGTTGGATCAGAAGAGTCTCTGTGTCGTCGGTCGGGCACAGG1530.16224470318763123No Hit
GTCTATGTATGAGACCAGCTGGGTGACTCGGTTAAACCCCTCGCCTCTCT1470.15588216580772413No Hit
ATTTAAAGCTGCAACACAGCTGGTGATGAGAGGCTTCCTCAGTCCAGTCG1380.14633835973786347No Hit
GTGGAGATGGTGTCCACGTCTTCCCTCCACCTGGAACCTGCATTCAATCC1360.1442175139445611No Hit
ATTTTAAATACCACAACTGGGGCAGAATTTAAAGCTGCAACACAGCTGGT1360.1442175139445611No Hit
CTTCTGATCCAACCCCTAGAACCGCGTTGGGTTTGTGGGTGTCTCGTGCT1360.1442175139445611No Hit
GATTTACCCTGAATCATTCTTCAGCAAGTACTAAAAAGGTATAATTTCTT1330.14103624525460753No Hit
GAGTACATGGGATCTCCCTCCGCCTCATTGCCTATTTTGTCCTCAGGTAC1290.1367945536680028No Hit
GTGCTAAGGAGCGACTGGACTGAGGAAGCCTCTCATCACCAGCTGTGTTG1290.1367945536680028No Hit
GTATAAAACTTGCATAGAGATTCCAGGAACACTTGTTCAAATGCAAATTC1270.13467370787470043No Hit
TACATAGACAGCACTTGTGAAGGATTGAATGCAGGTTCCAGGTGGAGGGA1270.13467370787470043No Hit
TTCTTCCTCAGTGCTGGGGGGTTGGAGACACCACCCCCTTCTGCTGCCCT1260.13361328497804922No Hit
ATTCTAGACGTGCCCTCGGCCACAGTGTCTAATCTTCCTGTTTCCTGGCA1250.13255286208139805No Hit
GCTCAGTGGAGATGGTGTCCACGTCTTCCCTCCACCTGGAACCTGCATTC1240.13149243918474687No Hit
GTTTAGAGAGGCGAGGGGTTTAACCGAGTCACCCAGCTGGTCTCATACAT1210.12831117049479335No Hit
CAATATATTTCGCTTAAAATAAGAAGCACGTGGAAAAAGAAAGACTTTGA1180.12512990180483977No Hit
ATCACATACTTTGTTTAGAGAGGCGAGGGGTTTAACCGAGTCACCCAGCT1170.12406947890818859No Hit
GTCAGATGTCTGGTGGCAGAGAGTCCGAGCATGGAGCGATTCCATTTTAT1150.12194863311488621No Hit
TACCACAACTGGGGCAGAATTTAAAGCTGCAACACAGCTGGTGATGAGAG1140.12088821021823504No Hit
CCCTAGAACCGCGTTGGGTTTGTGGGTGTCTCGTGCTCAGCCACTCTGCC1140.12088821021823504No Hit
GTACATGGGAGCCGGTGTAAATGTTGAGCCTTTTTGGCCTGGCTTGTTTG1100.11664651863163028No Hit
GTGCTGGGGGGTTGGAGACACCACCCCCTTCTGCTGCCCTCCTCTGGGCT1090.1155860957349791No Hit
ACAATATATTTCGCTTAAAATAAGAAGCACGTGGAAAAAGAAAGACTTTG1080.11452567283832793No Hit
TTTTATGACTGTTGTTTTTCACATTTTCATCTTTCTAAGGTGTGTCTCTT1070.11346524994167674No Hit
ATATATGACATTCTGGAAGAGGCAGTGTTGTACAGACAGAAAGCAAATCC1070.11346524994167674No Hit
GTATCAACGCAGAGTACATGGGATCTCCCTCCGCCTCATTGCCTATTTTG1050.11134440414837436No Hit
GAAACAAACAATATATTTCGCTTAAAATAAGAAGCACGTGGAAAAAGAAA1050.11134440414837436No Hit
TTCTCAGTGTGAGCCAATCTGGGACCCACTGCCCCACCCAGAAGTGGCCC1040.11028398125172319No Hit
GGCCTGGTAGCATGCCTGATGTTCCTTCTTTATTAAATGACGGAGTCAGG1020.10816313545842081No Hit
ACTTTTGGCTTGCAAAAATGACTTCTCATTGGAAAAGAGACACACCTTAG1010.10710271256176963No Hit
GTCCAGTCGCTCCTTAGCACCAGGCACCGTGGGTCCTGGATGGGGAACTG1000.10604228966511844No Hit
GTGCAGGTTGGGAAGGCTTCCTGGAGGTGACGGTCCCTAAGCTGAGCCTA990.10498186676846727No Hit
TCTCTAAACAAAGTATGTGATTTACCCTGAATCATTCTTCAGCAAGTACT990.10498186676846727No Hit
ACCCAGAAGTGGCCCAGTCCTGGGGGCAGGGGAGAAAGGGAGGGGTGAAA970.10286102097516489No Hit
ATCCAGGACCCACGGTGCCTGGTGCTAAGGAGCGACTGGACTGAGGAAGC970.10286102097516489No Hit
GTATGAGACCAGCTGGGTGACTCGGTTAAACCCCTCGCCTCTCTAAACAA960.10180059807851372No Hit
CTATGTATGAGACCAGCTGGGTGACTCGGTTAAACCCCTCGCCTCTCTAA960.10180059807851372No Hit
GATCAGAAGAGTCTCTGTGTCGTCGGTCGGGCACAGGCTTGCCTCCTCGA950.10074017518186254No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)