FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11035
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT346631.409152695967375No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT120610.928862709560489RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT8737.9111916628908014RNA PCR Primer, Index 26 (100% over 24bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG8647.829632985953783RNA PCR Primer, Index 26 (100% over 29bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT3623.2804712279111916RNA PCR Primer, Index 26 (100% over 27bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA2852.582691436338922RNA PCR Primer, Index 26 (100% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT2161.9574082464884457No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG1951.7671046669687356RNA PCR Primer, Index 26 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC1191.07838695061169RNA PCR Primer, Index 26 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT1131.0240144993203444RNA PCR Primer, Index 26 (100% over 21bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC1080.9787041232442228RNA PCR Primer, Index 26 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGT950.8608971454463071No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC860.7793384685092887No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTT690.6252831898504758RNA PCR Primer, Index 26 (95% over 22bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG530.48028998640688714No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC470.4259175351155414No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCT470.4259175351155414RNA PCR Primer, Index 26 (95% over 24bp)
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT430.38966923425464434RNA PCR Primer, Index 26 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGT410.37154508382419577No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.3534209333937472No Hit
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC360.32623470774807434RNA PCR Primer, Index 26 (95% over 22bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTCCTGAG350.31717263253285005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.31717263253285005No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTT310.2809243316719529RNA PCR Primer, Index 26 (95% over 22bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGA290.26280018124150434No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCT250.22655188038060714RNA PCR Primer, Index 26 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATATCGTATGCCGT220.19936565473493428No Hit
TCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCTTG210.19030357951971003RNA PCR Primer, Index 26 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1449932034435886No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCCT150.1359311282283643RNA PCR Primer, Index 26 (95% over 22bp)
TCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCTGCTTG150.1359311282283643RNA PCR Primer, Index 26 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC140.12686905301314No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGTCTTCT140.12686905301314RNA PCR Primer, Index 26 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTTAGCATCTCGTATGCCGT130.11780697779791571No Hit
CTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCTTGA120.10874490258269143RNA PCR Primer, Index 26 (96% over 29bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)