FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340647
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75062.203454015447076No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12740.3739941934025545No Hit
CATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTCGCCGT7130.20930758233596655Illumina RNA PCR Primer (95% over 24bp)
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG5410.1588154306364066No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGCTATAATTTTTCATCT4200.1232947890338092No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG3910.11478157741004619No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC3460.10157142144213804No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)