FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001421659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589460
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT214460.8282035636773689No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT93550.3612722343654662TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT66700.2575826620221977RNA PCR Primer, Index 3 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55320.21363527530836546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40760.15740733589242545No Hit
CAATAATACAGCACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACT39020.15068778818749856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28180.10882577834760915No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG27720.10704934619573192No Hit
TCCCTGAAGACTTCCTGTCTCTGAGCAGCTCAGTTCAGTTCCAGGTCATA25920.10009808994925584No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)