FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190651
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.3960115603904517No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT5630.2953039847679792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.2627838301398891No Hit
CAATAATACAGCACATAAAAGAGATTACATTAAGAGAAGAGACAGGAACT4580.24022952934943956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3650.19144929740730446No Hit
TCCCCATAGGGGACACTTATCCTTTGGCTAAACTAATATAAATAATGGAA3010.15788010553314696No Hit
GTCCAGGAGTACGTGTATGACCTGGAACTGAACTGAGCTGCTCAGAGACA2870.150536844810675No Hit
TTCTTACACCGCGAGGAAGCTTCCTCGCAACTTTGTGGTAGATTACTATG2580.1353258047426974No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT2420.12693350677415802RNA PCR Primer, Index 39 (95% over 23bp)
CAATAACACGGCACATAAAAAAGATTAAATTAAGAGAAGGGACAGGAACT2200.11539409706741639No Hit
CTCCTGGACCCAGGACTCACTGGGGTCAGCGCAGACTTGCTTGCCTCTTT2110.11067342946011298No Hit
TCCCTGAAGACTTCCTGTCTCTGAGCAGCTCAGTTCAGTTCCAGGTCATA2100.11014891083707928No Hit
TCTCTGAGCAGCTCAGTTCAGTTCCAGGTCATACACGTACTCCTGGACCC2000.10490372460674217No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)