FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417667
Sequences flagged as poor quality0
Sequence length100
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT4986211.938218724486253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT219715.260410805737585No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT197244.722422408282196TruSeq Adapter, Index 1 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160843.8509147239307873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142933.422104212207332No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT110862.6542676342636597RNA PCR Primer, Index 1 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63611.5229836209228882No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63271.5148431645305949No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50271.2035904201193774No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29180.6986426986091792Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24760.5928167655093651No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG24380.5837186083650372TruSeq Adapter, Index 1 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC23100.5530721842999328No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22070.528411389935044No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT19320.46256946323267095No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT13590.3253788305037266TruSeq Adapter, Index 1 (96% over 26bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC12880.308379642155114No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG12460.2983237842587516TruSeq Adapter, Index 1 (96% over 28bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.20806048837949848No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7930.1898641740908427No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7900.18914589852681682No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC7860.1881881977747823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6530.15634464776963466No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT6060.14509166393322911No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC5780.1383877586689875Illumina PCR Primer Index 5 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT5710.1367117823529271No Hit
GTACATGGGGAGGTCAAAGGTCAGAGGTAAAGAAGTCATGGCGGCGCTGT5680.1359935067889012No Hit
CTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC5550.13288097934478904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4960.11875489325227993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4760.11396638949210734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4730.11324811392808146No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4510.10798075979189163No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4460.1067836338518485No Hit
GCGTTGATACCACTGCTTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT4200.10055857896362413Clontech SMART CDS Primer II A (95% over 21bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)