FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001421703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217722
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT690593.1139610825883497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT592762.672832753609334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383491.729206816724549No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT316551.4273655579914886RNA PCR Primer, Index 39 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312811.4105014063980967No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT208470.9400186317311188RNA PCR Primer, Index 39 (96% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86350.3893635000238984No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG85550.3857561948702317RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT65410.2949422876266728RNA PCR Primer, Index 39 (96% over 28bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62560.2820912630167352Clontech SMART CDS Primer II A (100% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58250.26265690650135587No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56350.2540895567613975No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG53470.24110325820819742Illumina PCR Primer Index 11 (96% over 25bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43330.1953806653854721No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC35010.15786469178733853No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA33900.152859555886626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG33040.1489817028464343No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32370.14596058478023846No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30510.13757360029796342Clontech Universal Primer Mix Long (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT29250.13189209468093835RNA PCR Primer, Index 39 (95% over 22bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA28000.12625568037833418RNA PCR Primer, Index 39 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG24980.1126381034232424No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC22810.10285328819392152Illumina PCR Primer Index 11 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)