FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792952
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT587097.403852944440521No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT259043.2667803347491398RNA PCR Primer, Index 23 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209962.6478273590330814No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT165872.091803791402254RNA PCR Primer, Index 23 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156241.9703588615704357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131361.6565945984120096No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64920.8187128602992363No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG61250.7724301092626036RNA PCR Primer, Index 23 (96% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59390.7489734561486698No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45470.5734268909089074No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT43660.5506007929862086RNA PCR Primer, Index 23 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG42070.5305491379049425Illumina PCR Primer Index 1 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC28040.35361535124446375No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26810.3381036935400882Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25020.3155298176938831No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT23190.29245149769469025RNA PCR Primer, Index 23 (95% over 22bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.29119038731222063No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA22730.28665038993533026RNA PCR Primer, Index 23 (96% over 30bp)
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC16530.20846154622221774Illumina PCR Primer Index 1 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC14900.18790544698796396No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10780.1359476992302182Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC10190.12850714797364785RNA PCR Primer, Index 23 (95% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG9300.11728326556966878No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9290.11715715453142182No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)