FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001421707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971666
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT567595.841410525839126No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT241962.4901560824398508RNA PCR Primer, Index 31 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152571.57018975656244No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT140241.4432943007165013RNA PCR Primer, Index 31 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112041.153071117029926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96130.9893317250989537No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42260.43492311143952755No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30640.3153346931970451No Hit
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC26850.27632952063775No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26440.2721099637118104No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG26380.2714924675763071RNA PCR Primer, Index 38 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT21980.2262094176393946RNA PCR Primer, Index 31 (95% over 21bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19910.20490580096452896Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17790.18308760417674386No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15370.158181926711442No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC11540.11876509006181137No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACGC10090.10384226678714702No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)