FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001421708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971666
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA162801.6754728476657617No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17620.18133803179281768No Hit
CATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTCGCCGT12310.12668962380077106Illumina Single End PCR Primer 1 (95% over 24bp)
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG9880.10168103031288529No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)