FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227652
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80093.5180890130550138No Hit
CATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTCGCCGT10090.44322035387345593Illumina Single End PCR Primer 1 (95% over 24bp)
CTTATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTG8560.3760125103227734No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8530.37469470946883837No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC5960.26180310298174403No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCC5080.2231476112663188No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4970.21831567480189062No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC4820.21172667053221583No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC4690.20601620016516436No Hit
ATACACATCTGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTC4480.1967915941876197No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCC4290.18844552211269833No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2960.13002301758824872Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCCC2430.10674186916873121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)