FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68527
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT37185.425598669137712RNA PCR Primer, Index 46 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT34064.9703036759233585No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT23593.4424387467713458RNA PCR Primer, Index 46 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12651.8459877128722986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8571.2506019525150671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7511.0959183971281392No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG6390.9324791687947815TruSeq Adapter, Index 4 (95% over 23bp)
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT5670.8274110934376232RNA PCR Primer, Index 46 (96% over 26bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG5480.7996847957739285RNA PCR Primer, Index 46 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.6099785486012812No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC3790.553066674449487No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT3620.5282589344346024No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.5092883097173377No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.41297590730660905No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG2030.29623360135421073No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC1860.2714258613393261TruSeq Adapter, Index 18 (95% over 24bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA1800.2626701883928962RNA PCR Primer, Index 46 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1780.2597516307440863No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1540.2247289389583668Clontech SMART CDS Primer II A (100% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1180.17219490127978754No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.17219490127978754No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.16927634363097757No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1050.1532242765625228Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT880.12841653654763815RNA PCR Primer, Index 46 (95% over 21bp)
TCTTTATATCGAGCAAACATGACTCTTCGAACAGCATCTCGATAAGTCTC720.1050680753571585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)