FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68527
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20933.054270579479621No Hit
CATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT2370.3458490813839801Illumina RNA PCR Primer (100% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1950.2845593707589709No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC1070.15614283421133276No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT970.14155004596728296Clontech Universal Primer Mix Long (96% over 26bp)
TCTTTATATCGAGCAAACATGACTCTTCGAACAGCATCTCGATAAGTCTC830.12112014242561327No Hit
GTGTATTCCCTGCATTGAATGCCTGTCTGTTGACATTATCTGTAAAAATT760.11090519065477841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)