FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001421721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489326
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT505202.02946500378014No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308381.2388092198450504No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT203440.8172493277296746RNA PCR Primer, Index 26 (100% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190510.7653075571459905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184950.7429721940798434No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT117480.47193497356312514RNA PCR Primer, Index 26 (100% over 24bp)
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT70990.2851775942564373No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA67270.2702337901905978No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCCAG66990.26910898773402925No Hit
TCCTGGAAGAGAGCGCGGCCGCCACGCTGGTTTTGCATCTTCAGGAGACG63100.2534822678909874No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61590.24741636892877833No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60260.24207355726007762No Hit
GGCTACGAGCGTCTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTCT60240.24199321422746556No Hit
TTCCAGAGCCACATCATCGCGGTCGAAATAGAAGCCCAGAGAGAGGTAGG58420.2346819982597699No Hit
GAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCT55990.22492031979740704No Hit
GTACATGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACA55660.22359465975930834No Hit
TCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGGGC54540.21909544993303406No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53970.21680567350359092No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC50930.20459353254656082No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGG45800.18398554468157244No Hit
CTCCGGGACCATCTTCTCGGCCATCTCCTGCTTCTGGGACCTGCCAGCAC45610.18322228587175807No Hit
CCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCT45390.1823385125130256No Hit
TGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGG43500.17474609593118778No Hit
CTCTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCC39840.1600433209631844No Hit
GATGGGGGTCCGTGCGGGCAGAACCCAGGGCATGAAGATCCAAAAGGGCC39000.1566689135934787No Hit
GAGTACATGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAA37350.15004061340298538No Hit
TCCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGACC35660.143251627147268No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34810.13983704826125626Clontech SMART CDS Primer II A (100% over 21bp)
GTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCC34640.1391541324840539No Hit
GTATCAACGCAGAGTACATGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTC34370.13806950154379136No Hit
CATCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGG34080.13690452757091678No Hit
GCTCTTAGTCGTGCTTGAGAGTGAGCCTTTCGAAGAGATACTCGCCCAGC33390.13413269294580138No Hit
GTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCT32700.131360858320686No Hit
CTCGTAGCCCTCGCGCTTCTCCTCGGCCAGTTCGCGGAAGAAGTGGCTCA32390.13011554131519937No Hit
CTTCAGAAGTCGCTGGGCTCAGAAGGCTCTTAGTCGTGCTTGAGAGTGAG32080.12887022430971276No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG31180.12525478784217095RNA PCR Primer, Index 26 (96% over 27bp)
GCTTGAGAGTGAGCCTTTCGAAGAGATACTCGCCCAGCCCAGCCTCCGGG30990.12449152903235654No Hit
ATTTGTACCTGCAGGCCTCCTACACCTACCTCTCTCTGGGCTTCTATTTC30770.1236077556736241No Hit
GTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGGACTCTCTTC30490.12248295321705555No Hit
CTCCAGGGCCATGGCAGCTTTCATGGCGTCTGGGGTTTTACCCCACTCAT30400.12212140957030136No Hit
CTTCCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGA28220.11336401901558896No Hit
CTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTCTCTTCCAGGACAT27460.11031098377633143No Hit
CTTCCAGCCTCCGACCGCCCTCCGATTTCCTCTCCGCTTGCAACCTCCGG27260.10950755345021101No Hit
GAATTATTCCACCGACGTGGAGGCAGCCGTCAACAGCCTGGTCAATTTGT27120.10894515222192673No Hit
ACCATGAGCTCCCAGATTCGTCAGAATTATTCCACCGACGTGGAGGCAGC26800.10765966370013409No Hit
GTTTTACCCCACTCATCTTCAGCTGGCTTCTTGATGTCCTGGAAGAGAGC26770.10753914915121604No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG26640.10701691943923777RNA PCR Primer, Index 26 (100% over 29bp)
GCCCAGCCTCCGGGCCACCCAGCCTGTGGAGGTTGGTCAGGTGGTCACCC26340.10581177395005716No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26020.10452628542826452No Hit
GCTCTGGAAGGCGTGAGCCACTTCTTCCGCGAACTGGCCGAGGAGAAGCG25850.10384336965106218No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC25520.1025177096129635RNA PCR Primer, Index 26 (95% over 22bp)
CATGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG25470.1023168520314334No Hit
GTCACAGAGATGGGGGTCCGTGCGGGCAGAACCCAGGGCATGAAGATCCA25450.10223650899882136No Hit
GCTCAGAAGGCTCTTAGTCGTGCTTGAGAGTGAGCCTTTCGAAGAGATAC25250.10143307867270096No Hit
CTCTTCCAGCCTCCGACCGCCCTCCGATTTCCTCTCCGCTTGCAACCTCC25170.10111170654225281No Hit
CCCATCTTCTTGATAAGCTTCACTTCCTCATCTAGGAAGTGAGTCTCCAG24950.10022793318352037No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)