FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001421722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2489326
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA297311.194339351294286No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA69690.2799552971366547No Hit
CTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCCAG67380.27067567686996397No Hit
GGCTTAGGCAGAAGCCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCT63630.2556113582552064No Hit
GGCTACGAGCGTCTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTCT57930.23271359396077493No Hit
TCCTGGAAGAGAGCGCGGCCGCCACGCTGGTTTTGCATCTTCAGGAGACG57060.22921867204215118No Hit
TTCCAGAGCCACATCATCGCGGTCGAAATAGAAGCCCAGAGAGAGGTAGG55440.2227108864005759No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC53920.21660481592206082No Hit
CCCTATTACTTTGCAAGGGGCCCTTCAGAAGTCGCTGGGCTCAGAAGGCT48020.19290362130150893No Hit
GAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCCTCCGATTTCCT47830.19214036249169453No Hit
GTACATGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACA47640.19137710368188016No Hit
TGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGG47440.19057367335575975No Hit
TCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGGGC47360.1902523012253116No Hit
CTCCTACACCTACCTCTCTCTGGGCTTCTATTTCGACCGCGATGATGTGG45300.18197696886627143No Hit
CTCCGGGACCATCTTCTCGGCCATCTCCTGCTTCTGGGACCTGCCAGCAC39480.15859714637616767No Hit
CTCTTCCAGGACATCAAGAAGCCAGCTGAAGATGAGTGGGGTAAAACCCC38500.15466033777817773No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37580.15096455827802385No Hit
TCCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGACC36530.14674654906589174No Hit
GATGGGGGTCCGTGCGGGCAGAACCCAGGGCATGAAGATCCAAAAGGGCC36450.14642517693544355No Hit
GAGTACATGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAA36230.1455414035767111No Hit
CATCTAGGAAGTGAGTCTCCAGGAAGTCACAGAGATGGGGGTCCGTGCGG33550.1347754372066977No Hit
GTTAAGAAAGCTGCCTATTGGCTGGAGGGAGAGGCTTAGGCAGAAGCCCT33070.13284720442400874No Hit
ATTTGTACCTGCAGGCCTCCTACACCTACCTCTCTCTGGGCTTCTATTTC31290.12569667452153716No Hit
CTTCAGAAGTCGCTGGGCTCAGAAGGCTCTTAGTCGTGCTTGAGAGTGAG30590.12288466838011575No Hit
CTCCAGGGCCATGGCAGCTTTCATGGCGTCTGGGGTTTTACCCCACTCAT30340.12188038047246524No Hit
GTGTTTGGACGGAACAGATCCGGGGACTCTCTTCCAGCCTCCGACCGCCC30080.12083592104850871No Hit
CTCGTAGCCCTCGCGCTTCTCCTCGGCCAGTTCGCGGAAGAAGTGGCTCA28850.11589482454286823No Hit
GTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGGACTCTCTTC28390.11404693479279131No Hit
GTATCAACGCAGAGTACATGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTC28280.11360504811342508No Hit
CTTCCTAGATGAGGAAGTGAAGCTTATCAAGAAGATGGGTGACCACCTGA27600.11087338500461572No Hit
GCTCTTAGTCGTGCTTGAGAGTGAGCCTTTCGAAGAGATACTCGCCCAGC27530.11059218439047358No Hit
GAATTATTCCACCGACGTGGAGGCAGCCGTCAACAGCCTGGTCAATTTGT26960.10830240796103041No Hit
ACCATGAGCTCCCAGATTCGTCAGAATTATTCCACCGACGTGGAGGCAGC26510.10649468972725949No Hit
CTCCTGAAGATGCAAAACCAGCGTGGCGGCCGCGCTCTCTTCCAGGACAT25790.10360234055322606No Hit
GGGTAAAACCCCAGACGCCATGAAAGCTGCCATGGCCCTGGAGAAAAAGC25490.10239719506404545No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)