FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20514
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT599129.2044457443697No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT205810.032173150043873RNA PCR Primer, Index 23 (95% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG16878.223652140001949RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT14437.0342205323193925RNA PCR Primer, Index 23 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGT7443.6267914594910793No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA6803.3148093984595888RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT5892.8712099054304376RNA PCR Primer, Index 23 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG3411.6622794189334114Illumina PCR Primer Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGT2761.3454226381983034No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTT2261.1016866530174516No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC1950.9505703422053232No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC1870.9115725845763869No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT1860.9066978648727698RNA PCR Primer, Index 23 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCT1710.8335770693185143No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC1670.8140781905040461Illumina PCR Primer Index 1 (95% over 24bp)
TCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCTTG1270.6190894023593643Illumina Single End Adapter 1 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGT1050.5118455688797894No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG1040.5069708491761723No Hit
CTTTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC990.4825972506580871RNA PCR Primer, Index 23 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.4192258945110656No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTT810.39485229599298044No Hit
TCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAA770.3753534171785122Illumina PCR Primer Index 1 (96% over 27bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.3656039777712782No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGT670.3266062201423418No Hit
ATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTT660.3217315004387248Illumina PCR Primer Index 1 (96% over 26bp)
CTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCTTGA650.3168567807351077Illumina Single End Adapter 1 (96% over 25bp)
TCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCTGCTTG640.3119820610314907Illumina Single End Adapter 1 (100% over 25bp)
CTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC600.2924831822170225No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCT590.2876084625134055No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGA560.27298430340255436No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCAGAGAG400.19498878814468168No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAAATCGTATGCCGT390.19011406844106463No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCT390.19011406844106463Illumina Single End Adapter 1 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGTCTT390.19011406844106463No Hit
CTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCTGCTTGA380.1852393487374476Illumina Single End Adapter 1 (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.1852393487374476No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTG330.1608657502193624RNA PCR Primer, Index 23 (95% over 22bp)
CTCGGAGATGTGTATAAGAGACAGATCTCCGAGCCCACGAGACCAGAGAG290.14136687140489423No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTC290.14136687140489423No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGTCTTCT280.13649215170127718No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGTCTT280.13649215170127718No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.13161743199766013No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCTGCT260.12674271229404308Illumina Single End Adapter 1 (100% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGTCTTCTGCTTG250.12186799259042605Illumina Single End Adapter 1 (96% over 25bp)
TATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCT240.11699327288680901No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCC230.11211855318319197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)