FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12845
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT373329.061891786687426No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT130810.182950564421954RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT9427.333592837680031RNA PCR Primer, Index 33 (95% over 24bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG7796.064616582327754RNA PCR Primer, Index 33 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGT3852.997275204359673No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT3782.9427792915531334RNA PCR Primer, Index 33 (96% over 27bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA2602.024133904242896RNA PCR Primer, Index 33 (96% over 29bp)
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG2231.73608407940833RNA PCR Primer, Index 15 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC1471.1444141689373297No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGT1321.0276372129233164No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC1230.9575710393149086RNA PCR Primer, Index 15 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC1180.9186453873102374No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTT1170.9108602569093032No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT980.7629427792915531RNA PCR Primer, Index 33 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCT680.5293888672635267Illumina PCR Primer Index 4 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC590.4593226936551187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.4593226936551187No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC530.4126119112495134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.37368625924484233No Hit
CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC450.3503308680420397No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGT450.3503308680420397No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGTCTT420.32697547683923706No Hit
GTACATGGGTTAGGAATTGCATTCGATACTGGGAAGCTAGAGTATGGGAG410.31919034643830285No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT400.3114052160373686No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT370.288049824834566RNA PCR Primer, Index 15 (96% over 27bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGG350.2724795640326975No Hit
TCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCTTG340.26469443363176337Illumina PCR Primer Index 4 (96% over 26bp)
GTATTAGGCCAGATGGCTGCCTTCGCCATCGGTATTCCTCCAGATCTCTA340.26469443363176337No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACAAGAGGC300.23355391202802647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC290.22576878162709224No Hit
CCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGATGGCTGCCTTC280.21798365122615804No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGTCTTCT270.2101985208252238Illumina PCR Primer Index 4 (95% over 21bp)
CATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCT240.18684312962242117Illumina PCR Primer Index 4 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGTCTT220.17127286882055276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC220.17127286882055276No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAAATCGTATGCCGT210.16348773841961853No Hit
GTATCAACGCAGAGTACATGGGTTAGGAATTGCATTCGATACTGGGAAGC190.1479174776177501No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC190.1479174776177501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC180.1401323472168159No Hit
CTTTCGTACCTCAGCGTCAGTATTAGGCCAGATGGCTGCCTTCGCCATCG180.1401323472168159No Hit
CTTATAACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG180.1401323472168159No Hit
GGTTAGGAATTGCATTCGATACTGGGAAGCTAGAGTATGGGAGAGGATGG170.13234721681588169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.13234721681588169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC160.12456208641494744No Hit
ACGCAGAGTACATGGGTTAGGAATTGCATTCGATACTGGGAAGCTAGAGT160.12456208641494744No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.12456208641494744Clontech SMART CDS Primer II A (100% over 21bp)
GTATGGGAGAGGATGGTAGAATTCCAGGTGTAGCGGTGAAATGCGTAGAG160.12456208641494744No Hit
TCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGAA150.11677695601401324RNA PCR Primer, Index 15 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC150.11677695601401324No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCCCGTATGCCGT150.11677695601401324No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTG140.10899182561307902RNA PCR Primer, Index 48 (95% over 24bp)
TATCAACGCAGAGTACATGGGTTAGGAATTGCATTCGATACTGGGAAGCT140.10899182561307902No Hit
CTCGGAGATGTGTATAAGAGACAGATCTCCGAGCCCACGAGACAAGAGGC130.10120669521214481No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCATCTCGTATGCCGTC130.10120669521214481No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCT130.10120669521214481No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGC130.10120669521214481RNA PCR Primer, Index 48 (96% over 25bp)
ATACTGGGAAGCTAGAGTATGGGAGAGGATGGTAGAATTCCAGGTGTAGC130.10120669521214481No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG130.10120669521214481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)