FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7876
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT204525.964956830878617No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT7499.509903504316911TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG5176.564245810055866TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT5096.462671406805486RNA PCR Primer, Index 3 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGT2312.9329608938547485No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT2252.856780091416963TruSeq Adapter, Index 3 (96% over 26bp)
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA1592.01879126460132RNA PCR Primer, Index 3 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG1421.802945657694261TruSeq Adapter, Index 3 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGT791.0030472320975115No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTT770.9776536312849162No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT660.8379888268156425No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC630.7998984255967496Illumina PCR Primer Index 12 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCT570.7237176231589639Illumina PCR Primer Index 8 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT570.7237176231589639No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.6983240223463687No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC500.6348400203148806No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC430.5459624174707973No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.4570848146267141No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGT340.43169121381411885No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG330.41899441340782123No Hit
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC290.36820721178263077No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.36820721178263077No Hit
CTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC240.30472320975114275No Hit
TCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTG240.30472320975114275Illumina PCR Primer Index 8 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTT230.2920264093448451No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCT220.27932960893854747Illumina PCR Primer Index 8 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.26663280853224985No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAG200.25393600812595224No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT190.24123920771965465TruSeq Adapter, Index 3 (96% over 27bp)
CATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCT170.21584560690705942Illumina PCR Primer Index 8 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGT170.21584560690705942No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.20314880650076178Clontech SMART CDS Primer II A (100% over 21bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGTAGAGG150.1904520060944642No Hit
CTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTGA120.15236160487557138Illumina PCR Primer Index 8 (96% over 26bp)
GGTATCAACGCAGAGTACTTTGTTTGTTTTTTTTTTTTTTTTTTTTTTTT120.15236160487557138No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC120.15236160487557138No Hit
GTATCAACGCAGAGTACTTTGTTTGTTTTTTTTTTTTTTTTTTTTTGCTT110.13966480446927373No Hit
GTATCAACGCAGAGTACTTTGTTTGTTTTTTTTTTTTTTTTTTTTTTTTT110.13966480446927373No Hit
GTATCAACGCAGAGTACTTTGTTTGTTTTTTTTTTTTTTTTTTTTGCTTG100.12696800406297612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC100.12696800406297612No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGATCTCGTATGCCGTC90.11427120365667852No Hit
GGTATCAACGCAGAGTACTTTGTTTGTTTTTTTTTTTTTTTTTTTTGCTT90.11427120365667852No Hit
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA90.11427120365667852TruSeq Adapter, Index 3 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTC90.11427120365667852No Hit
TATCAACGCAGAGTACTTTGTTTGTTTTTTTTTTTTTTTTTTTTTGCTTG90.11427120365667852No Hit
TCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCTGCTTG90.11427120365667852Illumina PCR Primer Index 8 (96% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.10157440325038089No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGTCTT80.10157440325038089No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAAATCGTATGCCGT80.10157440325038089No Hit
ACTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG80.10157440325038089No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC80.10157440325038089No Hit
ACGCAGAGTACTTTGTTTGTTTTTTTTTTTTTTTTTTTTTGCTTGGCGAA80.10157440325038089No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTG80.10157440325038089Illumina PCR Primer Index 8 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)