FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18387
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT562730.6031435253168No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT193410.518300973513895TruSeq Adapter, Index 11 (95% over 21bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG17609.571980203404578TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT13267.211616903246859RNA PCR Primer, Index 11 (95% over 23bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA6173.3556316963071735RNA PCR Primer, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT5883.1979115679556207TruSeq Adapter, Index 11 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGT4432.409310926197857No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG3271.7784304127916464TruSeq Adapter, Index 11 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGT1760.9571980203404579No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC1710.9300048947626041No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1690.9191276445314623No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTT1660.9028117691847501No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT1640.8919345189536085No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC1610.8756186436068961RNA PCR Primer, Index 17 (95% over 24bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCT1080.5873715124816447Illumina PCR Primer Index 9 (95% over 21bp)
TCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTGCTTG1000.5438625115570783Illumina PCR Primer Index 9 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGT860.4677217599390874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.4514058845923751No Hit
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA780.42421275901452116TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.4187741338989503No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA760.4133355087833796No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC720.39158100832109644No Hit
CTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC570.3100016315875347No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT560.3045630064719639TruSeq Adapter, Index 11 (96% over 27bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACT490.2664926306629684No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGT470.25561538043182686No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTT460.25017675531625605No Hit
CTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTGCTTGA400.21754500462283136Illumina PCR Primer Index 9 (96% over 26bp)
TCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTTCTGCTTG370.20122912927611902Illumina Single End Adapter 1 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTTCT290.15772012835155275Illumina Single End Adapter 1 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGTCTT290.15772012835155275No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGTACTA280.15228150323598194No Hit
CATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTGCT270.14684287812041116Illumina PCR Primer Index 9 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC260.14140425300484039No Hit
TCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGTCTTCTGCTTG240.1305270027736988Illumina PCR Primer Index 9 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC240.1305270027736988No Hit
CATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTTCTGCT210.11421112742698646Illumina Single End Adapter 1 (96% over 25bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCCTG210.11421112742698646Illumina PCR Primer Index 9 (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.11421112742698646Clontech SMART CDS Primer II A (100% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGTCTTCT200.10877250231141568Illumina PCR Primer Index 9 (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)