FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6910
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT229633.227206946454416No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT81611.808972503617946RNA PCR Primer, Index 26 (100% over 22bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG5908.5383502170767RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT4967.1780028943560055RNA PCR Primer, Index 26 (100% over 24bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT2383.444283646888567RNA PCR Primer, Index 26 (100% over 27bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA1932.793053545586107RNA PCR Primer, Index 26 (100% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT1311.8958031837916063No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG1211.7510853835021707RNA PCR Primer, Index 26 (96% over 27bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC721.0419681620839363No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGT690.9985528219971056No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC640.9261939218523878RNA PCR Primer, Index 26 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT550.7959479015918958RNA PCR Primer, Index 26 (100% over 21bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC500.723589001447178RNA PCR Primer, Index 26 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCT320.4630969609261939RNA PCR Primer, Index 26 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG300.43415340086830684No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGT290.4196816208393632No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTT280.40520984081041966RNA PCR Primer, Index 26 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTT260.37626628075253254RNA PCR Primer, Index 26 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC240.3473227206946454No Hit
CTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC240.3473227206946454RNA PCR Primer, Index 26 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.3473227206946454No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT180.26049204052098407RNA PCR Primer, Index 26 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.26049204052098407No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCT170.24602026049204054RNA PCR Primer, Index 26 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATATCGTATGCCGT160.23154848046309695No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGA160.23154848046309695No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCT150.21707670043415342RNA PCR Primer, Index 26 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTTAGCATCTCGTATGCCGT120.1736613603473227No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGTCTT120.1736613603473227RNA PCR Primer, Index 26 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1736613603473227No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTCCTGAG100.1447178002894356No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG100.1447178002894356No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTTCTGCT100.1447178002894356RNA PCR Primer, Index 26 (96% over 27bp)
TCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAA90.13024602026049203RNA PCR Primer, Index 26 (96% over 31bp)
ACTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG80.11577424023154848No Hit
TCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCTGCTTG80.11577424023154848RNA PCR Primer, Index 26 (96% over 29bp)
CTTATCACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC70.10130246020260492RNA PCR Primer, Index 26 (95% over 22bp)
TATCACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT70.10130246020260492RNA PCR Primer, Index 26 (100% over 22bp)
CTTATAACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG70.10130246020260492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)