FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20891
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT714534.201330716576514No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT248611.899861184242019RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT17608.424680484419127RNA PCR Primer, Index 27 (96% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG16607.946005456895314RNA PCR Primer, Index 27 (96% over 30bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT7093.3938059451438423RNA PCR Primer, Index 27 (96% over 28bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA5212.4938968933990715RNA PCR Primer, Index 27 (96% over 30bp)
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG4171.9960748647743047RNA PCR Primer, Index 27 (100% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGT4071.9482073620219234No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC2471.1823273179838207No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT2181.0435115600019147RNA PCR Primer, Index 27 (95% over 22bp)
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC1950.9334163036714374TruSeq Adapter, Index 27 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC1930.9238428031209611No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGT1700.813747546790484No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTT1460.6988655401847685No Hit
CTTTACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC1210.579196783303815RNA PCR Primer, Index 27 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCT1090.5217557800009573RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGT1010.4834617777990522No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTCC960.4595280264228615No Hit
CTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC860.4116605236704801No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.33985926954190804No Hit
ATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTT700.33507251926666987RNA PCR Primer, Index 27 (100% over 27bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.306352017615241No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGGACTC570.272844765688574No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTT560.2680580154133359No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGGACTCC480.22976401321143075No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTTCT460.2201905126609545RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATATCGTATGCCGT420.20104351156000191No Hit
TCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCTGCTTG400.19147001100952563RNA PCR Primer, Index 38 (96% over 26bp)
CATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGTCTTCTGCT320.1531760088076205RNA PCR Primer, Index 38 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCCT300.1436025082571442RNA PCR Primer, Index 27 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGTCTT280.13402900770666795No Hit
CTTATAACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG240.11488200660571538No Hit
TCTCCGAGCCCACGAGACGGACTCCTAGCTCGTATGCCGTCTTCTGCTTG240.11488200660571538RNA PCR Primer, Index 38 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGG230.11009525633047725No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1053085060552391No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTACCTCGTATGCCGTCTTCT210.10052175578000096RNA PCR Primer, Index 38 (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)