FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275723
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT85333.0947726522633223No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT29151.057220471270079RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG25910.9397112319247941RNA PCR Primer, Index 39 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT19420.7043300703967388RNA PCR Primer, Index 39 (96% over 25bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA9310.3376577216989515RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT8300.3010267551129213RNA PCR Primer, Index 39 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.2542406690772986No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.20564116885424866No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG4490.16284459403096585Illumina PCR Primer Index 11 (96% over 25bp)
GTCTTGCTCAGAGGCCGGCACTCGCCCACTGTGACGATGTCACCGATCTG4140.15015069471897521No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGT3460.12548826176996478No Hit
GCCTTACACCGTGCTGACCTGGGTATCATCCATGTGCTCTGAAGCATCCA3440.1247628960949939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.11460777664540137No Hit
GGTGTACAGACATGTTCTTGTGGCGCTTCTCGAAGCGGTTGTACTTGCGG2900.10517802287077972No Hit
CTGGTAATGTGTCCATTCGAGGGCGGATCCTCTCTGGCGTGGTGACCAAG2820.10227656017089615No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)