FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268931
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT2719110.11077190803589No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT116324.325273025422878RNA PCR Primer, Index 23 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT83293.097076945387479RNA PCR Primer, Index 23 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78632.9237982977046157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54022.0086936797914707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41871.5569049310046073No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG26600.9891012936403761RNA PCR Primer, Index 23 (96% over 30bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG24780.9214259419702452Illumina PCR Primer Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT18960.7050135536624637RNA PCR Primer, Index 23 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC12110.45030137842048706No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11500.4276189803332453No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT9610.3573407305219554RNA PCR Primer, Index 23 (95% over 22bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA9130.33949228612543736RNA PCR Primer, Index 23 (96% over 30bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.30788566583993665Clontech SMART CDS Primer II A (100% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7680.28557511034428906No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC6910.2569432307915413Illumina PCR Primer Index 1 (95% over 24bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.2565713881999472No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5710.21232211980024615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5250.19521736058691633No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG4930.1833183976559043No Hit
CTTTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC4840.17997181433155718RNA PCR Primer, Index 23 (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4690.17439417545764527Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGT4450.16546995325938624No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC4120.15319914773678006No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.13906912925620327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3720.138325444073015No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGT3390.12605463855040885No Hit
CTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC3390.12605463855040885No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3300.1227080552260617Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT3150.11713041635214982No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3100.11527120339417918No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGT3060.11378383302780266No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)