FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21484
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT487922.709923664122137No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT18638.671569540122883TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT15207.075032582386892RNA PCR Primer, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG9174.2682926829268295TruSeq Adapter, Index 11 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT4732.2016384285980264TruSeq Adapter, Index 11 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG4071.894433066468069TruSeq Adapter, Index 11 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3901.8153044125861106No Hit
GTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT3511.6337739713274995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2761.2846769689070936No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA2311.0752187674548501RNA PCR Primer, Index 11 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC2080.968162353379259No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.9169614596909327No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT1800.837832805808974No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC1610.749394898529138RNA PCR Primer, Index 17 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA1350.6283746043567305No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.5352820703779557No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1140.5306274436790169No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.5166635635822007Clontech SMART CDS Primer II A (100% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC920.4282256563023646No Hit
GTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT730.3397877490225284RNA PCR Primer, Index 11 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC690.3211692422267734No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG690.3211692422267734TruSeq Adapter, Index 3 (96% over 28bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG620.2885868553342022RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG580.2699683485384472RNA PCR Primer, Index 23 (96% over 30bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG570.26531372183950847RNA PCR Primer, Index 33 (96% over 29bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA560.26065909514056973Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG550.256004468441631TruSeq Adapter, Index 1 (96% over 28bp)
GCACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT550.256004468441631TruSeq Adapter, Index 11 (95% over 23bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.25134984174269226No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.25134984174269226No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT480.22342208154905976No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGT470.21876745485012103No Hit
GACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG450.2094582014522435TruSeq Adapter, Index 11 (95% over 24bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG430.20014894805436603RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG420.1954943213554273RNA PCR Primer, Index 17 (96% over 28bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT410.19083969465648853Clontech Universal Primer Mix Long (96% over 26bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGTACTA400.1861850679575498No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACT400.1861850679575498No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG330.15360268106497857RNA PCR Primer, Index 39 (96% over 30bp)
GATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT330.15360268106497857RNA PCR Primer, Index 17 (96% over 25bp)
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT320.14894805436603983TruSeq Adapter, Index 11 (96% over 27bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG310.1442934276671011RNA PCR Primer, Index 26 (100% over 29bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG310.1442934276671011Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.13963880096816234No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.13032954757028486No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC280.13032954757028486Clontech SMART CDS Primer II A (96% over 26bp)
GTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC250.11636566747346862No Hit
GCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG250.11636566747346862TruSeq Adapter, Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG240.11171104077452988RNA PCR Primer, Index 46 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGT240.11171104077452988No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG240.11171104077452988TruSeq Adapter, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA230.10705641407559115RNA PCR Primer, Index 23 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)