FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55878
Sequences flagged as poor quality0
Sequence length100
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT1010118.076881778159564No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT41027.340992877339919TruSeq Adapter, Index 1 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32345.787608718994954No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT32215.764343748881492RNA PCR Primer, Index 1 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18383.2893088514263216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16622.974336948351766No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9401.6822363005118295No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8761.5677010630301729No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8261.4782204087476287No Hit
GTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT7611.3618955581803214No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG7341.3135760048677476TruSeq Adapter, Index 1 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC5070.9073338344249973No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG5060.9055442213393464TruSeq Adapter, Index 1 (96% over 28bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT4200.7516374959733706TruSeq Adapter, Index 1 (96% over 26bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCAT3920.7015283295751459No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT3910.699738716489495No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.6263645799778088No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.5547800565517735No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.5064605032391997Clontech SMART CDS Primer II A (100% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC2090.3740291349010344No Hit
GTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT1920.3436057124449694RNA PCR Primer, Index 1 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC1740.3113926769032535No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGC1650.2952861591323956Illumina PCR Primer Index 5 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC1560.2791796413615376No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG1510.27023157593328323TruSeq Adapter, Index 3 (96% over 28bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA1420.25412505816242525RNA PCR Primer, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG1090.19506782633594616RNA PCR Primer, Index 33 (96% over 29bp)
GCACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT1090.19506782633594616TruSeq Adapter, Index 1 (95% over 23bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG1040.18611976090769175RNA PCR Primer, Index 31 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT980.17538208239378647No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.17359246930813557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC950.17001324313683383No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.16822363005118293No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.16464440387988116No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG810.14495865993772147RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG780.1395898206807688RNA PCR Primer, Index 27 (96% over 30bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAGGCA770.13780020759511794No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG770.13780020759511794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC760.13601059450946706No Hit
GATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.13601059450946706No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG740.1324313683381653RNA PCR Primer, Index 39 (96% over 30bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA690.12348330290991089Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATATCGTATGCCGT690.12348330290991089No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG670.1199040767386091RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG670.1199040767386091RNA PCR Primer, Index 23 (96% over 30bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT660.11811446365295823Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT660.11811446365295823No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGAACTCGTATGCCGT640.11453523748165646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC570.10200794588210028No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)