FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87477
Sequences flagged as poor quality0
Sequence length100
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT1215913.899653623238109No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT50605.784377607828343RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT45185.164786172365307RNA PCR Primer, Index 39 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40094.5829189386924565No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG32333.6958286178081097RNA PCR Primer, Index 39 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22432.564102564102564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20282.31832367365136No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT16001.8290522080089622RNA PCR Primer, Index 39 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14181.620997519347943No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12701.4518101901071139No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12311.4072270425368956No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG11361.2986270676863632Illumina PCR Primer Index 11 (96% over 25bp)
GTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT10481.1980291962458705No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA9141.0448460738251197RNA PCR Primer, Index 39 (96% over 30bp)
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC5960.6813219474833385Illumina PCR Primer Index 11 (96% over 26bp)
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC5530.6321661693930977No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCTA4740.5418567166226551No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT4720.5395704013626439RNA PCR Primer, Index 39 (95% over 22bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.5155640911325263No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4120.4709809435623078No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.4698377859323022Clontech SMART CDS Primer II A (100% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG3750.4286841112521005TruSeq Adapter, Index 3 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC2920.3338020279616356RNA PCR Primer, Index 39 (95% over 21bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG2680.3063662448415012RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG2510.28693256513140597RNA PCR Primer, Index 23 (96% over 30bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG2480.28350309224138914RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG2390.2732146735713387RNA PCR Primer, Index 27 (96% over 30bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC2370.27092835831132756No Hit
GTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT2370.27092835831132756RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG2170.24806520571121551TruSeq Adapter, Index 1 (96% over 28bp)
GCACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT2090.2389199446711707RNA PCR Primer, Index 39 (96% over 25bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.19205048184094106No Hit
CTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC1590.18176206317089064No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1540.17604627502086262Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1540.17604627502086262Clontech Universal Primer Mix Long (96% over 26bp)
GACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG1510.17261680213084582RNA PCR Primer, Index 39 (96% over 26bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCTCTCTA1400.1600420682007842No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG1310.1497536495307338RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG1280.14632417664071698RNA PCR Primer, Index 17 (96% over 28bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCTCTCT1210.13832207323067777No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.13489260034066097No Hit
GCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG1160.13260628508064978Illumina PCR Primer Index 11 (96% over 29bp)
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTT1150.13146312745064417Illumina PCR Primer Index 11 (96% over 28bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG1140.13031996982063856TruSeq Adapter, Index 10 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC1070.12231786641059936No Hit
GATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT1070.12231786641059936Illumina PCR Primer Index 11 (96% over 27bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.12117470878059376No Hit
GATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060.12117470878059376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT1050.12003155115058814No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG1050.12003155115058814RNA PCR Primer, Index 46 (96% over 28bp)
GTGCTGGGATTACAAGCATAAGCCACCGTGCCCAGCCTCAAAATGCTATT990.11317260537055454No Hit
GTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC970.11088629011054335No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG920.10517050196051535Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAA910.10402734433050972Illumina PCR Primer Index 11 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG890.10174102907049853No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)