FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101282
Sequences flagged as poor quality0
Sequence length100
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT1427514.094310933828321No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT60165.939851108785372RNA PCR Primer, Index 23 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT52005.13417981477459RNA PCR Primer, Index 23 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41374.084634979562015No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG29622.9245078098773725RNA PCR Primer, Index 23 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23012.271874568037756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20532.027013684563891No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT15441.5244564680792245RNA PCR Primer, Index 23 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13571.3398234632017536No Hit
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG12891.272684188700855Illumina PCR Primer Index 1 (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12711.2549120278035584No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11851.1700005924053631No Hit
GTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT10791.0653423115657275No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA8940.8826839912324006RNA PCR Primer, Index 23 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC7230.7138484627080823No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC5680.5608104105369167Illumina PCR Primer Index 1 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT5460.5390888805513319RNA PCR Primer, Index 23 (95% over 22bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG4850.47886100195493775No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.4739242905945775No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.44627870697656047No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.4265318615351198Clontech SMART CDS Primer II A (100% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC3270.3228609229675559RNA PCR Primer, Index 23 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC2950.2912659702612508No Hit
GTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT2550.25177227937836927RNA PCR Primer, Index 23 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC2030.2004304812306234No Hit
GCACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT1750.17278489761260638RNA PCR Primer, Index 23 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGT1720.16982287079639027No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.15896210580359788No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1530.15106336762702158Clontech Universal Primer Mix Long (96% over 26bp)
GACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG1480.14612665626666138RNA PCR Primer, Index 23 (96% over 26bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG1430.1411899449063012TruSeq Adapter, Index 3 (96% over 28bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1430.1411899449063012Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1340.1323038644576529No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGT1290.1273671530972927No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT1290.1273671530972927No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGA1270.12539246855314864No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCAGAGAG1240.12243044173693252No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1150.1135443612882842No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1150.1135443612882842No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.1105823344720681No Hit
GCCATGTATCACGTGGAGTTGCTCCTTACCACACCTCACGTGCCCCTGAG1120.1105823344720681No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG1070.10564562311170791RNA PCR Primer, Index 33 (96% over 29bp)
ATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTT1060.10465828083963587Illumina PCR Primer Index 1 (96% over 26bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG1020.10070891175134773RNA PCR Primer, Index 31 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)