FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54869
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18073.2932985838998343No Hit
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCCGT5781.0534181413913137Illumina RNA PCR Primer (96% over 25bp)
CTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTG5370.9786947092164975No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5250.9568244363848439No Hit
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTC3240.5904973664546466No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1510.2752009331316408Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1170.21323516010862237Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.17131713718128633No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG690.12575406878200807Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.11481893236618126No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG620.11299640963021013Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT600.10935136415826786No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT560.10206127321438335No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)