FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37541
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT709218.89134546229456No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT28337.5464159185956685TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT24806.6061106523534265RNA PCR Primer, Index 10 (95% over 23bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG11162.972749793559042TruSeq Adapter, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT7822.0830558589275725TruSeq Adapter, Index 10 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6791.808689166511281No Hit
GTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT5951.5849338057057616No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG5321.4171172851016223TruSeq Adapter, Index 10 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4831.286593324631736No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC3791.0095628779201407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.857728883087824No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA3120.8310913401347859RNA PCR Primer, Index 10 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT2740.7298686769132415No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC2580.6872486081883806Illumina Paired End PCR Primer 2 (95% over 24bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT2430.6472922937588237No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.495458298926507Clontech SMART CDS Primer II A (100% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1640.43685570442982335No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC1440.38358061852374736No Hit
GTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT1320.35161556698010177RNA PCR Primer, Index 10 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC1320.35161556698010177No Hit
GCACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT990.26371167523507627TruSeq Adapter, Index 10 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC970.2583841666444687No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.22109160651021548No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.2131003436243041No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.1997815721477851No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT600.1598252577182281No Hit
GACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG600.1598252577182281TruSeq Adapter, Index 10 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGT580.15449774912762046No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGT510.13585146906049386No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGAGGCT500.13318771476519006No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG460.12253269758397485TruSeq Adapter, Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGT450.11986894328867106No Hit
GATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT450.11986894328867106Illumina PCR Primer Index 9 (96% over 25bp)
GTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC430.11454143469806345No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGC430.11454143469806345No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT420.11187768040275967Clontech Universal Primer Mix Long (96% over 26bp)
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT420.11187768040275967TruSeq Adapter, Index 10 (96% over 27bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.10388641751684824No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA380.10122266322154444Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG380.10122266322154444RNA PCR Primer, Index 27 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)