FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37541
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5511.4677286167123944No Hit
CATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTCGCCGT3680.9802615806717988Illumina RNA PCR Primer (96% over 25bp)
CTTATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTG3450.9189952318798114No Hit
ATACACATCTGACGCTGCCGACGATCTTACGCGTGTAGATCTCGGTGGTC2190.5833621906715325No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.33563304120827897No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA490.13052396046988626Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT410.10921392610745584Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)