FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281892
Sequences flagged as poor quality0
Sequence length100
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT4710316.70959090715593No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT165895.884877896499368TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT114374.057227590708498RNA PCR Primer, Index 3 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104073.6918394278659914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68902.444198487363955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62252.208292537567579No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG27310.9688107502163948TruSeq Adapter, Index 3 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26310.9333361712996467No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC20510.7275836135825068No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT15130.5367303790104011No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13740.48742071431612105Clontech SMART CDS Primer II A (100% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.4590410511827224No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC10880.38596341861422107No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG9740.34552239864912804No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.3203354476182368No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8980.31856171867239935No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.28308713975565114No Hit
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC7720.27386374923729656No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT6940.24619357768223293TruSeq Adapter, Index 3 (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5710.20255984561463256Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG5540.19652916719878535TruSeq Adapter, Index 3 (96% over 28bp)
CTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC4530.16069984249286962No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4240.15041221460701262Clontech Universal Primer Mix Long (96% over 26bp)
GCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG4230.15005746881784512No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG4150.14721950250450527Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3970.14083407829949057No Hit
GTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT3910.1387056035644857No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.1199040767386091No Hit
GATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT3280.11635661884693428No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC3130.11103543200942205Illumina PCR Primer Index 12 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT3060.10855221148524967No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT3030.10748797411774721Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT3010.10677848253941226No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)