FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281892
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87993.1214081988846796No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12390.43953003277851094No Hit
CATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT7860.2788301902856413Illumina RNA PCR Primer (100% over 25bp)
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG6870.24371035715806053No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5080.18021086089708116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4120.14615526513700283Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4020.142607807245328No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC3960.1404793325103231No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC3550.12593475515445632No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC3420.12132305989527904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCC3220.11422814411192939No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG3130.11103543200942205Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC3120.11068068622025456No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)