FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001421837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964074
Sequences flagged as poor quality0
Sequence length100
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT604223.076360666655126No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT595773.0333378477593005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385021.9603131042924045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328791.6740204289655076No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT241251.228314208120468RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT156700.7978314462693361RNA PCR Primer, Index 27 (96% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104540.5322610044224403No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62170.31653593500041244No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60750.3093060648427707Clontech SMART CDS Primer II A (100% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57960.29510089742036194No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48820.24856497260286525No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36080.18369979949838958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG34320.17473883366919984No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG31110.15839525394664355RNA PCR Primer, Index 27 (100% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC25410.1293739441589268No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT24550.12499529040148183RNA PCR Primer, Index 27 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG22440.11425231432216913No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)