FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504143
Sequences flagged as poor quality0
Sequence length100
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT413948.210765596269312No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205274.071662206953186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160273.1790583227377947No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT142942.8353066491055117TruSeq Adapter, Index 1 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136092.699432502286058No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT86401.7137994576935514RNA PCR Primer, Index 1 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66861.326211015525357No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54481.080645769156767No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47450.9412012068004514No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28380.56293551631184Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27240.5403228845783835No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22310.44253317015211957No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTG17090.33899111958313416TruSeq Adapter, Index 1 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC15740.3122130030566724No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCT15350.30447710272680567No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC11190.22196083254156065No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.18228954879865436No Hit
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCT8790.1743552920500731TruSeq Adapter, Index 1 (96% over 26bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG8660.1717766586067842TruSeq Adapter, Index 1 (96% over 28bp)
GTACTGGGCAGGTGGCCGGCTGTCCCTCAGGCACCACAACGCCGAGCCAC8470.16800788665120808No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8320.16503254037049012No Hit
GTATTGGCCCAGCTGTAATCAACTCAGCAGGCTTTTGGTTGTAAATTCAA8290.1644374711143465No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7930.15729664004062338Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7880.15630485794705073Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCAGTACTGGTCAGCTTTTCAACACTATTCCCTTTGACCTACTGGCCA7830.1553130758534781No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7670.15213937315404558No Hit
ACGCAGAAGAGTGGAGGTGTGAGCAGAGACTGGATTGGCGTCCCCAGGAA7350.14579196775518058No Hit
GTTCAGAGACATCTGGATTTGCTGTATCCATACAAGCAAAAGCTTTTCCA7080.14043634444988823No Hit
GCTTTAATGTCATGCTAACCAGTTACCTTGTTAGAGCTGGGAAGCCACCT6370.1263530387211565No Hit
GTACATGGGCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC6260.12417111811529664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6260.12417111811529664No Hit
CTTTACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC5910.11722864346028807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5820.11544343569185728No Hit
GTATAAGCCAGGTGCTAAGGAGCCCCGCTGCGTGTGTGTGAGAACCACCG5790.11484836643571368No Hit
ATACAGTACGAGGCAATAACAAATTAGTGTGGGTTGATTCTGGAATTGGA5650.11207137657371023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT5410.10731082252456148No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)