FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001421840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504143
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA249674.952364706045706No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37970.7531593218590757No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC10150.20133176499524935No Hit
GTATTGGCCCAGCTGTAATCAACTCAGCAGGCTTTTGGTTGTAAATTCAA10030.19895148797067497No Hit
GTACTGGGCAGGTGGCCGGCTGTCCCTCAGGCACCACAACGCCGAGCCAC9990.19815806229581687No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9970.19776134945838778Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC8750.17356186637521498No Hit
GCCCAGTACTGGTCAGCTTTTCAACACTATTCCCTTTGACCTACTGGCCA8740.17336350995650043No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC8250.1636440454394884No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC8110.16086705557748496No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCC7500.14876731403589855No Hit
GTTCAGAGACATCTGGATTTGCTGTATCCATACAAGCAAAAGCTTTTCCA6560.1301218106767326No Hit
GTATAAGCCAGGTGCTAAGGAGCCCCGCTGCGTGTGTGTGAGAACCACCG6430.1275431772334437No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6230.12357604885915305Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGTATTGGCCCAGCTGTAATCAACTCAGCAGGCTTTTGGTTGTAAA6220.12337769244043853No Hit
GTACATGGGCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCAC5930.11762535629771712No Hit
CATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTCGCCGT5830.11564179211057181Illumina Single End PCR Primer 1 (100% over 23bp)
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG5750.11405494076085554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5570.11048452522399398No Hit
GCTTTAATGTCATGCTAACCAGTTACCTTGTTAGAGCTGGGAAGCCACCT5500.10909603029299227No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCCC5330.10572397117484522No Hit
GTACACAGGCTGCCCACCGCTAGCCATCACATGCTCCTTTCCACTCTAAG5100.10116177354441101No Hit
ACGCAGAAGAGTGGAGGTGTGAGCAGAGACTGGATTGGCGTCCCCAGGAA5070.10056670428826742No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)