FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353941
Sequences flagged as poor quality0
Sequence length100
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT4178111.804509791179886No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT181915.13955715783139TruSeq Adapter, Index 10 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157144.439723004681571No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT137253.8777649382241672RNA PCR Primer, Index 10 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107923.04909575324701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106453.0075634074605655No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG51391.4519369047383603TruSeq Adapter, Index 10 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43871.2394721154090655No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT36091.019661469001896TruSeq Adapter, Index 10 (96% over 26bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG31690.8953469646070956TruSeq Adapter, Index 10 (95% over 24bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24540.6933358949655452No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23690.669320592980186No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.5622405994219376Clontech SMART CDS Primer II A (100% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18260.5159051932384211No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT17380.49104229235946106No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC16980.47974097377811553No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA15720.4441418202468773RNA PCR Primer, Index 10 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14370.4059998700348363No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC14040.39667628220522627Illumina Paired End PCR Primer 2 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC8420.23789275613732233No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC8150.23026436609491413No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7750.21896304751356865No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT6430.18166869619512857No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6240.17630056986898945No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5800.16386911942950944No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5510.15567566345803396No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5460.15426299863536577Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5180.14635207562842395No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4850.13702848779881394Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC4770.13476822408254482No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT4290.12120664178493026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4060.11470838360065659No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4040.11414331767158933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3860.10905772430998388No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT3800.10736252652278205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3710.1048197298419793No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)