FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431532
Sequences flagged as poor quality0
Sequence length100
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT4690010.868255424858411No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT223045.168562238721578RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT149533.4650964470769257RNA PCR Primer, Index 33 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125012.896888295653625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96142.227876495833449No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79411.8401879814243207No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43481.0075730189186434No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG33350.7728279710427037RNA PCR Primer, Index 15 (95% over 24bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25810.598101647154788No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24790.5744649295996589No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC22230.5151414031867857No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT20950.48547963998034904RNA PCR Primer, Index 33 (95% over 21bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG18990.440060065070493RNA PCR Primer, Index 33 (96% over 29bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT16400.38004134108246895RNA PCR Primer, Index 33 (96% over 27bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15740.3647469944291501No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15500.3591854138279432No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14560.3374025564732164Clontech SMART CDS Primer II A (100% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC9640.22339015414847568No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9390.21759684102221852Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9300.21551124829676596Clontech Universal Primer Mix Long (96% over 26bp)
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC8660.20068036669354766No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC7760.1798244394390219RNA PCR Primer, Index 15 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC6920.16035890733479788No Hit
ATATAACAGTTTGCTAACAGGATTTTTGACTATTCACTTTGGGAGTTATT6780.15711465198409388No Hit
GTCCTAAATCGGGGTAATTTGGGGAAGATTCTATCACTTTCTCTGGAATG6300.14599149078168017No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.1258307611023053No Hit
ATTCTAGGTAGTGCCCTATTTTACAATTGTAAAACTGAGGCACGAGCAAA5390.12490383100210413No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.12281823827665155No Hit
ATATATAACAGTTTGCTAACAGGATTTTTGACTATTCACTTTGGGAGTTA5280.12235477322655099No Hit
GTCTTACTACATACCAGGCACTGTACTTGGCCATCTAGGTAACTAAGAAA5020.11632972757524354No Hit
CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC4930.11424413484979097No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.11354893727464012No Hit
CCTAAGTACCATGCATTCAGATTATGGTTTCAAGTTCAAATAACAGCTAA4450.10312097364737725No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)