FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001421850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431532
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA142403.299871156716072No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19200.444926448096549No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10140.23497678040098996Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTCGCCGT7590.17588498651316703Illumina Single End PCR Primer 1 (100% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7410.1717138010622619Clontech Universal Primer Mix Long (96% over 26bp)
ATATAACAGTTTGCTAACAGGATTTTTGACTATTCACTTTGGGAGTTATT6570.1522482689580379No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC5960.13811258492997044No Hit
GTCCTAAATCGGGGTAATTTGGGGAAGATTCTATCACTTTCTCTGGAATG5870.13602699220451786No Hit
ATATATAACAGTTTGCTAACAGGATTTTTGACTATTCACTTTGGGAGTTA5740.13301446937886416No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG5710.13231927180371328No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCC5550.1286115514029087No Hit
GTCTTACTACATACCAGGCACTGTACTTGGCCATCTAGGTAACTAAGAAA5340.12374516837685269No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC5220.12096437807624927No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5090.11795185525059555Clontech Universal Primer Mix Long (96% over 26bp)
ATTCTAGGTAGTGCCCTATTTTACAATTGTAAAACTGAGGCACGAGCAAA5070.11748839020049498No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC4780.1107681469740367No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCCCC4770.1105364144489864No Hit
CCTAAGTACCATGCATTCAGATTATGGTTTCAAGTTCAAATAACAGCTAA4350.10080364839687439No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)