FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18539
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT536428.93359943902044No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT189910.243270942337775RNA PCR Primer, Index 23 (95% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG16849.083553589729759RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT13387.217217757160581RNA PCR Primer, Index 23 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGT6483.495334160418577No Hit
CTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGA6203.344301202869626RNA PCR Primer, Index 23 (96% over 30bp)
CATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCT5803.1285398349425537RNA PCR Primer, Index 23 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTG3381.823183558983764Illumina PCR Primer Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGT2651.4294190625168564No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTT2231.2028696261934302No Hit
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC1700.9169858136900587No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCT1610.8684395059064675No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGC1540.8306812665192297Illumina PCR Primer Index 1 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCC1520.8198931981228761No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCT1430.7713468903392848RNA PCR Primer, Index 23 (95% over 22bp)
TCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCTTG1020.5501914882140353Illumina Single End Adapter 1 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGT890.4800690436377366No Hit
CTTTACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC850.45849290684502936RNA PCR Primer, Index 23 (95% over 21bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGAG810.4369167700523221No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGT780.4207346674577917No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTT780.4207346674577917No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCT660.3560062570796699No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.3398241544851394No Hit
CTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTC610.3290360860887858No Hit
TCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTGAA590.31824801769243216Illumina PCR Primer Index 1 (96% over 27bp)
TCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCTGCTTG560.3020659150979017Illumina Single End Adapter 1 (100% over 25bp)
ATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTT560.3020659150979017Illumina PCR Primer Index 1 (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.2858838125033713No Hit
CTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCTTGA510.27509574410701765Illumina Single End Adapter 1 (96% over 25bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCAGAGAG470.25351960731431034No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGCT460.24812557311613354Illumina Single End Adapter 1 (95% over 23bp)
CACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTG380.20497329953071902RNA PCR Primer, Index 23 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGTCTT360.1941852311343654No Hit
CTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCTGCTTGA350.1887911969361886Illumina Single End Adapter 1 (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAAATCGTATGCCGT330.17800312853983494No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGAGA280.15103295754895085No Hit
TATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCT270.14563892335077405No Hit
GGCTCGGAGATGTGTATAAGAGACAGACTCCGAGCCCACGAGACCAGAGA260.14024488915259722No Hit
CATCTCCGAGCCCACGAGACCAGAGAGGAGCTCGTATGCCGTCTTCTGCT240.1294568207562436Illumina Single End Adapter 1 (100% over 23bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGACCTCGTATGCCGTCTTCT230.12406278655806677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.11866875235988995No Hit
ACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTCTTCTGC210.11327471816171314RNA PCR Primer, Index 23 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCATAGAGGATCTCGTATGCCGT210.11327471816171314No Hit
TATCACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT200.10788068396353633RNA PCR Primer, Index 23 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCCGTC200.10788068396353633No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCAGAGAGGAACTCGTATGCC190.10248664976535951No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATATCGTATGCCGTCTT190.10248664976535951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)