FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23317
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT847036.32542779945962No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT291912.518763134193936RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT19378.307243641977955RNA PCR Primer, Index 31 (95% over 24bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG13345.721147660505211RNA PCR Primer, Index 31 (96% over 29bp)
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT8763.7569155551743365RNA PCR Primer, Index 31 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGT5742.461723206244371No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG4882.0928935969464337RNA PCR Primer, Index 38 (95% over 24bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA3731.5996912124201228RNA PCR Primer, Index 31 (96% over 29bp)
ACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGC2701.1579534245400351RNA PCR Primer, Index 38 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC2691.1536647081528497No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCC2631.1279324098297379No Hit
TATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCT2401.0292919329244758RNA PCR Primer, Index 31 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGT2070.8877642921473602No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTT2010.8620319938242483No Hit
CTTTACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC1320.5661105631084616No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTACCTCGTATGCCGT1090.4674700862031994No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTTCT960.4117167731697903RNA PCR Primer, Index 38 (95% over 22bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACGC780.3345198782004546No Hit
CTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC770.3302311618132693No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGTCTT760.32594244542608397No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCCATCTCGTATGCCGT690.29592143071578675No Hit
ATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTT670.28734399794141613RNA PCR Primer, Index 38 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTAGCTCGTATGCCGTCTTCT520.2230132521336364RNA PCR Primer, Index 38 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.21872453574645107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.18441480464896856No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATATCGTATGCCGT390.1672599391002273No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTACCTCGTATGCCGTCTT360.15439378993867137No Hit
TCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTTCTGCTTG330.1415276407771154RNA PCR Primer, Index 38 (96% over 27bp)
CACATCTCCGAGCCCACGAGACGCTACGCTAACTCGTATGCCGTCTTCTG330.1415276407771154RNA PCR Primer, Index 27 (95% over 22bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGCTACGC310.13295020800274476No Hit
ACTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCG290.12437277522837414No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCCTCT270.11579534245400352RNA PCR Primer, Index 38 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCCT270.11579534245400352No Hit
CTTAATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCG270.11579534245400352No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTACG250.10721790967963289No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCCTG250.10721790967963289RNA PCR Primer, Index 38 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTACGCCATCTCGTATGCCGTCTT240.10292919329244757RNA PCR Primer, Index 41 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)