FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8312
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT246629.667949951876803No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT86510.406641000962463TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG6818.192974013474496TruSeq Adapter, Index 10 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT5857.038017324350338RNA PCR Primer, Index 10 (95% over 23bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA2523.031761308950914RNA PCR Primer, Index 10 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT2513.019730510105871TruSeq Adapter, Index 10 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGT1942.3339749759384025No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG1491.7925890279114534TruSeq Adapter, Index 10 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC800.9624639076034648No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC780.9384023099133783Illumina Paired End PCR Primer 2 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGT770.926371511068335No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTT700.8421559191530318No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT670.8060635226179018No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC600.7218479307025986No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCT450.541385948026949Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGT440.5293551491819056No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT370.44513955726660254No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC350.4210779595765159No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.3970163618864293No Hit
TCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCTTG310.37295476419634266Illumina PCR Primer Index 5 (96% over 26bp)
CTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC270.32483156881616937No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGCT250.3007699711260828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.28873917228103946No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTT230.27670837343599614No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGAA210.2526467757459095TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCTGCTTG210.2526467757459095Illumina PCR Primer Index 5 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.2285851780558229No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCT180.21655437921077958Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGAGGCCGATCTCGTATGCCGT160.192492781520693No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTT150.18046198267564964TruSeq Adapter, Index 10 (96% over 27bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGAGGC130.15640038498556302No Hit
CCCCATGGGTTCTCTCCTTCCTCTGAACTTCTTTAGGAGTCACTGCTTGT130.15640038498556302No Hit
CTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCTTGA130.15640038498556302Illumina PCR Primer Index 5 (96% over 26bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGAGGCT120.14436958614051973No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTGCT120.14436958614051973Illumina PCR Primer Index 5 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGTCTT110.1323387872954764No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGTCTTCTG100.1203079884504331Illumina PCR Primer Index 5 (95% over 22bp)
CTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCTGCTTGA100.1203079884504331Illumina PCR Primer Index 5 (96% over 26bp)
CATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGTCTTCTGCT100.1203079884504331Illumina PCR Primer Index 5 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGTCTT100.1203079884504331No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAAATCGTATGCCGT90.10827718960538979No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGTCTTCT90.10827718960538979Illumina PCR Primer Index 5 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC90.10827718960538979No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)