FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16674
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT448926.922154252129065No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT16629.967614249730119RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT10706.417176442365359RNA PCR Primer, Index 33 (95% over 24bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG9835.895406021350605RNA PCR Primer, Index 33 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGT4422.6508336332013913No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCT4352.6088521050737676RNA PCR Primer, Index 33 (96% over 27bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA3532.1170684898644594RNA PCR Primer, Index 33 (96% over 29bp)
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG2731.63727959697733RNA PCR Primer, Index 15 (95% over 24bp)
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCT1510.9056015353244573RNA PCR Primer, Index 33 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTT1500.8996041741633681No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGT1410.8456279237135661No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC1350.8096437567470314No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGC1290.7736595897804965RNA PCR Primer, Index 15 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC1200.7196833393306945No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCT880.5277677821758426Illumina PCR Primer Index 4 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC680.40782055895406016No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGT640.38383111430970374No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATATCGTATGCCGT590.35384430850425813No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGGC540.3238575026988125No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGTCTT520.3118627803766343No Hit
TCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCTTG470.2818759745711887Illumina PCR Primer Index 4 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.2818759745711887No Hit
ATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT460.27587861341009956RNA PCR Primer, Index 15 (96% over 27bp)
CTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC450.26988125224901044No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACAAGAGG410.24589180760465393No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACAAGAGGC350.20990764063811923No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.19191555715485187No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAAGCTCGTATGCCGTCTTCT310.18591819599376275Illumina PCR Primer Index 4 (95% over 21bp)
CATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCT290.1739234736715845Illumina PCR Primer Index 4 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAAAATCGTATGCCGT240.1439366678661389No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTG240.1439366678661389RNA PCR Primer, Index 48 (95% over 24bp)
GTACATGGGATGTGCCATAATATACAAACTTCTACTTTCGTCAGTCCTTA230.13793930670504978No Hit
ATATAAAACAATTACCCTTGTGAAATAGAAATTCATGAAAATTCAGAGAG220.13194194554396066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.13194194554396066No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAGGCAATCTCGTATGCCGT220.13194194554396066No Hit
CTTATCACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC210.12594458438287154No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAACCTCGTATGCCGTCTT210.12594458438287154No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCATCTCGTATGCCGTC190.11394986206069328No Hit
CTCCGAGCCCACGAGACAAGAGGCAAACTCGTATGCCGTCTTCTGCTTGA170.10195513973851504Illumina PCR Primer Index 4 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)