FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16666
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT506130.367214688587545No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT184011.040441617664706TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG12927.752310092403696TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT12057.230289211568462RNA PCR Primer, Index 3 (95% over 23bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT5543.324132965318613TruSeq Adapter, Index 3 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGT5093.0541221648865955No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA4252.550102004080163RNA PCR Primer, Index 3 (96% over 28bp)
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG2831.6980679227169087TruSeq Adapter, Index 3 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGT1921.1520460818432738No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC1811.0860434417376694Illumina PCR Primer Index 12 (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTT1811.0860434417376694No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1671.0020400816032642No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT1550.9300372014880595No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCT1400.8400336013440538Illumina PCR Primer Index 8 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC1370.8220328813152527No Hit
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC710.4260170406816272No Hit
TCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTG690.4140165606624265Illumina PCR Primer Index 8 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.4080163206528261No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTT660.3960158406336254No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG650.39001560062402496No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGT640.3840153606144246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.3840153606144246No Hit
CTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC570.34201368054722187No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGT480.28801152046081846No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTT470.28201128045121804TruSeq Adapter, Index 3 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCT420.2520100804032161Illumina PCR Primer Index 8 (95% over 21bp)
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACGTAGAGG380.22800912036481458No Hit
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAG330.1980079203168127No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCT310.1860074402976119Illumina PCR Primer Index 8 (95% over 24bp)
TCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCTGCTTG310.1860074402976119Illumina PCR Primer Index 8 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.1800072002880115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC250.1500060002400096No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTG240.14400576023040923Illumina PCR Primer Index 8 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGTCTT230.13800552022080884No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAAATCGTATGCCGT230.13800552022080884No Hit
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGAA230.13800552022080884TruSeq Adapter, Index 3 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGATCTCGTATGCCGTC220.13200528021120844No Hit
CTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCTTGA220.13200528021120844Illumina PCR Primer Index 8 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCCT210.12600504020160805No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCT210.12600504020160805No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.11400456018240729Clontech SMART CDS Primer II A (100% over 21bp)
GGCTGATACAGAGTTTATTGAATTAGATTTTTCTATTTACAACTGAGATC190.11400456018240729No Hit
GATACAGAGTTTATTGAATTAGATTTTTCTATTTACAACTGAGATCACAT180.10800432017280691No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCCTCT180.10800432017280691Illumina PCR Primer Index 8 (95% over 21bp)
CATCTCCGAGCCCACGAGACGTAGAGGAAGCTCGTATGCCGTCTTCTGCT180.10800432017280691Illumina PCR Primer Index 8 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)