FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10254
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT278227.130875755802613No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT9479.235420323776088RNA PCR Primer, Index 46 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT7136.953384045250634RNA PCR Primer, Index 46 (95% over 23bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG5945.792861322410767RNA PCR Primer, Index 46 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGT2952.8769260776282426No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT2692.6233664911254144RNA PCR Primer, Index 46 (96% over 26bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA1731.6871464794226643RNA PCR Primer, Index 46 (96% over 28bp)
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG1501.462843768285547TruSeq Adapter, Index 4 (95% over 23bp)
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT1031.0044860542227423No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTT980.9557245952798908No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC970.9459723034913204TruSeq Adapter, Index 18 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGT960.9362200117027502No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC940.9167154281256095No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC780.7606787595084844No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.7411741759313439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.6631558416227814No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCT560.5461283401599376Illumina PCR Primer Index 8 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT450.4388531304856641No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC440.42910083869709387No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGCG430.4193485469085235No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAACCTCGTATGCCGT400.39009167154281255No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGTCTT350.341330212599961No Hit
TCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCTGCTTG290.2828164618685391Illumina PCR Primer Index 8 (96% over 26bp)
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTT270.26331187829139846TruSeq Adapter, Index 4 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGTCTTCT260.25355958650282817Illumina PCR Primer Index 8 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC260.25355958650282817No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC260.25355958650282817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC260.25355958650282817No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCTGCT240.23405500292568754Illumina PCR Primer Index 8 (95% over 24bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTAAGGC240.23405500292568754No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGT220.21455041934854693No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTAAGGCG210.20479812755997662No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAACCTCGTATGCCGTCTT190.18529354398283598No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC170.16578896040569535No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.14628437682855472Clontech SMART CDS Primer II A (100% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCCT150.14628437682855472No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGTCTTCTGCT140.1365320850399844Illumina PCR Primer Index 8 (95% over 24bp)
CTTATCACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC140.1365320850399844No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGATCTCGTATGCCGTC130.12677979325141409RNA PCR Primer, Index 33 (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.12677979325141409No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAAACTCGTATGCCGTCTTCTG130.12677979325141409RNA PCR Primer, Index 46 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAAAATCGTATGCCGT120.11702750146284377No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATATCGTATGCCGTCTT120.11702750146284377No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC120.11702750146284377No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCCTCT120.11702750146284377Illumina PCR Primer Index 8 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.11702750146284377No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.11702750146284377No Hit
TCTCCGAGCCCACGAGACTAAGGCGAAGCTCGTATGCCGTCTTCTGCTTG110.10727520967427347Illumina PCR Primer Index 8 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGG110.10727520967427347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC110.10727520967427347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)