FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9320
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT261828.09012875536481No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT8729.356223175965665TruSeq Adapter, Index 11 (95% over 21bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG8088.669527896995708TruSeq Adapter, Index 11 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT5916.341201716738197RNA PCR Primer, Index 11 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT2853.0579399141630903TruSeq Adapter, Index 11 (96% over 26bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA2642.832618025751073RNA PCR Primer, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGT2002.1459227467811157No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG1581.6952789699570814TruSeq Adapter, Index 11 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC961.0300429184549356No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC880.944206008583691RNA PCR Primer, Index 17 (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.9334763948497854No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC780.8369098712446352No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT750.8047210300429184No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGT750.8047210300429184No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTT680.7296137339055794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.5793991416309012No Hit
TCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTGCTTG440.4721030042918455Illumina PCR Primer Index 9 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCT440.4721030042918455Illumina PCR Primer Index 9 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.40772532188841204No Hit
TCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTTCTGCTTG350.37553648068669526Illumina Single End Adapter 1 (96% over 27bp)
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAA300.3218884120171674TruSeq Adapter, Index 11 (96% over 28bp)
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC290.3111587982832618No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGT280.30042918454935624No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTT230.24678111587982834No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGT210.22532188841201717No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACTA210.22532188841201717No Hit
CTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC200.2145922746781116No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTT190.20386266094420602TruSeq Adapter, Index 11 (96% over 27bp)
CTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTGCTTGA190.20386266094420602Illumina PCR Primer Index 9 (96% over 26bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTTCT170.18240343347639484Illumina Single End Adapter 1 (95% over 22bp)
GGAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCGTACT170.18240343347639484No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGTCTT150.1609442060085837No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.15021459227467812Clontech SMART CDS Primer II A (100% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC140.15021459227467812No Hit
TCTCCGAGCCCACGAGACCGTACTAGACCTCGTATGCCGTCTTCTGCTTG130.13948497854077252Illumina PCR Primer Index 9 (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC130.13948497854077252No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC120.12875536480686695No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACCGTACTA100.1072961373390558No Hit
CATCTCCGAGCCCACGAGACCGTACTAGAACTCGTATGCCGTCTTCTGCT100.1072961373390558Illumina PCR Primer Index 9 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)