FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8670
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC4114.740484429065743Illumina Single End PCR Primer 1 (95% over 21bp)
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT190.21914648212226065Illumina Single End PCR Primer 1 (96% over 26bp)
TATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGT170.19607843137254902No Hit
CACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCC130.14994232987312572Illumina Single End PCR Primer 1 (95% over 24bp)
ACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGC100.11534025374855825Illumina Single End PCR Primer 1 (95% over 23bp)
TTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGG100.11534025374855825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)