FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33809
Sequences flagged as poor quality0
Sequence length100
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT393411.63595492324529No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT14204.200065071430684RNA PCR Primer, Index 26 (100% over 22bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG10223.022863734508563RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT9572.830607234759975RNA PCR Primer, Index 26 (100% over 24bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT4071.203821467656541RNA PCR Primer, Index 26 (100% over 27bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA3421.0115649679079535RNA PCR Primer, Index 26 (100% over 29bp)
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG2620.7749415836019995RNA PCR Primer, Index 26 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGT2180.6447987222337247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.5619805377266408No Hit
GTACATGGGGGGGCGTGGGTTGAGGAGAGGCTCCAGACCCGCACGCCGCG1820.5383181992960454No Hit
CCTTAAATGTGTCCAAAAACAGTTCTGAGTGAGACTGTAATTTACAAAAT1810.535360406992221No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.45845780709278594No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC1360.40225975332012187No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGT1220.3608506610665799No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC1220.3608506610665799RNA PCR Primer, Index 26 (95% over 22bp)
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGC1150.3401461149398089RNA PCR Primer, Index 26 (96% over 28bp)
TCCCAATACAGTATTTCTTTCTTAAAAATAAATTCAACAAGTTTGCTGAA1140.33718832263598447No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT990.29282143807861816RNA PCR Primer, Index 26 (100% over 21bp)
GTCTTGGAGAGAGTGTAGTTATAACCATCCTTTCCAGCATACTTCTGGAA940.27803247655949603No Hit
GAATAAAAGAGAGCAAGATTAGACTTTATATTTCAGAGCCTTGGTCTAAC910.2691590996480227No Hit
CTCCAAGACAGAGTTCCTAAGCTTCATGAATACAGAACTAGCTGCCTTCA880.2602857227365495No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTT870.257327930432725RNA PCR Primer, Index 26 (95% over 22bp)
GTCAAGGACACCAGGGTCCTTCTGGTTCTTTGTGAAGGCAGCTAGTTCTG850.25141234582507616No Hit
GCCTTGAGGAAGGAGTCATGGCAAGCCATAGCTAGGCCACCAATCAGATT840.24845455352125173No Hit
GCCATAGCTAGGCCACCAATCAGATTAAGAAATTCTGAGAAATCTAGCTG810.23958117660977846No Hit
CATTTAAGGTGTAGTATTAATAGGTTAAAACCAGGCTTTCTAGAAAGAAT800.23662338430595403No Hit
GTCCTTGACCGCATGATGAAGAAACTGGACACCAACAGTGATGGTCAGCT730.21591883817918306No Hit
CTTTACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC730.21591883817918306No Hit
GTCTTTATACTGCAAACTATTTAAAGAAATATGTATTCTGTAAAAGAAAA720.21296104587535863No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT710.21000325357153424No Hit
GTTATAACCATCCTTTCCAGCATACTTCTGGAAGACAGCAATCAGGGACT690.20408766896388536No Hit
ATACTACACCTTAAATGTGTCCAAAAACAGTTCTGAGTGAGACTGTAATT690.20408766896388536No Hit
GCTCCAACATGGCAAAAATCTCCAGCCCTACAGAGACTGAGCGGTGCATC690.20408766896388536No Hit
TAACTACACTCTCTCCAAGACAGAGTTCCTAAGCTTCATGAATACAGAAC670.1981720843562365No Hit
GAATATGCATCTTAAAATGGCAAGTTTTCCATATTTTTACAACTCACTGG670.1981720843562365No Hit
CTTCTGGAAGACAGCAATCAGGGACTCGATGCACCGCTCAGTCTCTGTAG650.19225649974858766No Hit
GCCCTACAGAGACTGAGCGGTGCATCGAGTCCCTGATTGCTGTCTTCCAG640.18929870744476324No Hit
GTATAAAGACTAAACAAAGCTAGAGGGGTACTGTATCTTTAAGGCAGTAC630.18634091514093878No Hit
CTGTAGGGCTGGAGATTTTTGCCATGTTGGAGCTGAGCGAGGCGCGGGAG620.1833831228371144No Hit
GAGTACATGGGGGGGCGTGGGTTGAGGAGAGGCTCCAGACCCGCACGCCG610.18042533053328996No Hit
GTATTAATTTGTAAGAATATGCATCTTAAAATGGCAAGTTTTCCATATTT610.18042533053328996No Hit
GCCTTAAAGATACAGTACCCCTCTAGCTTTGTTTAGTCTTTATACTGCAA610.18042533053328996No Hit
TTGTACACCCATGAAAGAAAACTTTTATGCAAGGTCTTGCATTTAAAAGA600.1774675382294655No Hit
GTACATGGGATTACCAGAGGAAAAATAAACATTTCTCAGTAACTTTGCCT580.1715519536218167No Hit
GCTTTATTACTATTGGCAGGTGGGGCCTGCATGAGGTGGTTAGTGTGCTC570.16859416131799224No Hit
GGTTATAACTACACTCTCTCCAAGACAGAGTTCCTAAGCTTCATGAATAC550.1626785767103434No Hit
GCATTAATACGCCCATTATGGTCATTTAAATTGGGGTTTATTTCAGCAAA550.1626785767103434No Hit
GTATCAACGCAGAGTACATGGGGGGGCGTGGGTTGAGGAGAGGCTCCAGA540.15972078440651896No Hit
GTCTAACTCCCTTCAGTTTCGGTTGGTATCAGTTATGTTTTCTAACCAGA540.15972078440651896No Hit
GGATCACACTCATTGATGAAGAAAACTGTAACAGCACAGAATAAAAGAGA540.15972078440651896No Hit
ACATGGGGGGGCGTGGGTTGAGGAGAGGCTCCAGACCCGCACGCCGCGCG540.15972078440651896No Hit
GTATTAATAGGTTAAAACCAGGCTTTCTAGAAAGAATAAACTTACATATT540.15972078440651896No Hit
TTCTGGTTCTTTGTGAAGGCAGCTAGTTCTGTATTCATGAAGCTTAGGAA540.15972078440651896No Hit
GTCTTGCATTTAAAAGACAGCTTTGCGAATATTTTGTAAATTACAGTCTC530.15676299210269456No Hit
CCTCAATATTTTACTTTGAGAGGACATTAATTTCCATTTCAAAATAAAAT530.15676299210269456No Hit
GATTAAGAAATTCTGAGAAATCTAGCTGACCATCACTGTTGGTGTCCAGT520.1538051997988701No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACTCCTGAG520.1538051997988701No Hit
ATTAATACTACACCTTAAATGTGTCCAAAAACAGTTCTGAGTGAGACTGT520.1538051997988701No Hit
CTTTAAGGCAGTACCAATGAATCAATACATTTATAAAATGGAAAAGGGTT510.1508474074950457No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCAACTCGTATGCCGTCTTCT500.1478896151912213RNA PCR Primer, Index 26 (95% over 24bp)
AGATTAGACTTTATATTTCAGAGCCTTGGTCTAACTCCCTTCAGTTTCGG480.1419740305835724No Hit
TCACTGTTGGTGTCCAGTTTCTTCATCATGCGGTCAAGGACACCAGGGTC470.13901623827974802No Hit
CTTTTATTCTGTGCTGTTACAGTTTTCTTCATCAATGAGTGTGATCCAGT470.13901623827974802No Hit
CTTTATAACATTGCTTCTTGTTAAAACATTGTTATCAAGTAACAGTAACT450.13310065367209914No Hit
GTGTAGTTATAACCATCCTTTCCAGCATACTTCTGGAAGACAGCAATCAG450.13310065367209914No Hit
ATATTTTACTTTGAGAGGACATTAATTTCCATTTCAAAATAAAATATCTT450.13310065367209914No Hit
GTGCATCGAGTCCCTGATTGCTGTCTTCCAGAAGTATGCTGGAAAGGATG450.13310065367209914No Hit
CTCCAGACCCGCACGCCGCGCGCACAGAGCTCTCAGCGCCGCTCCCAGCC440.13014286136827474No Hit
TATTAATGCTATGGTTATCAATCTCCAAGAATATTGCTATTTTCATGTCC440.13014286136827474No Hit
CTTTGTACACCCATGAAAGAAAACTTTTATGCAAGGTCTTGCATTTAAAA440.13014286136827474No Hit
AGTTTATTCTTTCTAGAAAGCCTGGTTTTAACCTATTAATACTACACCTT440.13014286136827474No Hit
GTACAAAGGTTCCAAGGGACAGGACCAAGAACGAGGGGCTGAGACATTTA430.1271850690644503No Hit
ATGCAAGACCTTGCATAAAAGTTTTCTTTCATGGGTGTACAAAGAATGCG430.1271850690644503No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCACCTCGTATGCCGT430.1271850690644503No Hit
CTTTTCATGAGGATCGTCTGGTTAGAAAACATAACTGATACCAACCGAAA430.1271850690644503No Hit
GTACATGGGAAGACAGTGTTTGACGAAGCGATCCGAGCAGTCCTCTGCCC420.12422727676062587No Hit
TATTAATACTACACCTTAAATGTGTCCAAAAACAGTTCTGAGTGAGACTG420.12422727676062587No Hit
ACGCAGAGTACATGGGGGGGCGTGGGTTGAGGAGAGGCTCCAGACCCGCA420.12422727676062587No Hit
ATACAGAACTAGCTGCCTTCACAAAGAACCAGAAGGACCCTGGTGTCCTT410.12126948445680145No Hit
CTATTAATACTACACCTTAAATGTGTCCAAAAACAGTTCTGAGTGAGACT410.12126948445680145No Hit
TCTTAAAATGGCAAGTTTTCCATATTTTTACAACTCACTGGTGGTTTTCC410.12126948445680145No Hit
GTATCAACGCAGAGTACATGGGATTACCAGAGGAAAAATAAACATTTCTC400.11831169215297702No Hit
GGGTTGAGGAGAGGCTCCAGACCCGCACGCCGCGCGCACAGAGCTCTCAG400.11831169215297702No Hit
GTTTTAACCTATTAATACTACACCTTAAATGTGTCCAAAAACAGTTCTGA400.11831169215297702No Hit
GATAGAGGGTTTTTATTGAAAGTAGGTTATGCAAACTTGGCTTGAAAGGT380.11239610754532815No Hit
GTTCTGTATTCATGAAGCTTAGGAACTCTGTCTTGGAGAGAGTGTAGTTA380.11239610754532815No Hit
TTATATTTCAGAGCCTTGGTCTAACTCCCTTCAGTTTCGGTTGGTATCAG380.11239610754532815No Hit
ATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTT380.11239610754532815RNA PCR Primer, Index 26 (96% over 30bp)
TTCTTTGTACACCCATGAAAGAAAACTTTTATGCAAGGTCTTGCATTTAA370.10943831524150374No Hit
GTCCAAAAACAGTTCTGAGTGAGACTGTAATTTACAAAATATTCGCAAAG370.10943831524150374No Hit
GGTATCAACGCAGAGTACATGGGGGGGCGTGGGTTGAGGAGAGGCTCCAG370.10943831524150374No Hit
GTTGAGGAGAGGCTCCAGACCCGCACGCCGCGCGCACAGAGCTCTCAGCG370.10943831524150374No Hit
CGGAGATGTGTATAAGAGACAGACATCTCCGAGCCCACGAGACTCCTGAG360.10648052293767932No Hit
TATCAACGCAGAGTACATGGGGGGGCGTGGGTTGAGGAGAGGCTCCAGAC360.10648052293767932No Hit
GTACATGGGAAAGTTTAATTGAAAGAATGTAAAGCAACCAAAAAGAAAAA350.10352273063385488No Hit
CTTTCAGTGCTCCTCAATATTTTACTTTGAGAGGACATTAATTTCCATTT350.10352273063385488No Hit
AGCTATGGCTTGCCATGACTCCTTCCTCAAGGCTGTCCCTTCCCAGAAGC350.10352273063385488No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.10352273063385488Clontech SMART CDS Primer II A (100% over 21bp)
GACCATCACTGTTGGTGTCCAGTTTCTTCATCATGCGGTCAAGGACACCA350.10352273063385488No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCAGCTCGTATGCCGTCTT340.10056493833003047RNA PCR Primer, Index 26 (95% over 22bp)
CTGTTGGTGTCCAGTTTCTTCATCATGCGGTCAAGGACACCAGGGTCCTT340.10056493833003047No Hit
GGGCCAAGGGGTCCTCAGGTCCGCTTCTGGGAAGGGACAGCCTTGAGGAA340.10056493833003047No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)