FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33809
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAAATGTGTCCAAAAACAGTTCTGAGTGAGACTGTAATTTACAAAAT1880.5560649531189921No Hit
GTACATGGGGGGGCGTGGGTTGAGGAGAGGCTCCAGACCCGCACGCCGCG1660.49099352243485467No Hit
CTCCAAGACAGAGTTCCTAAGCTTCATGAATACAGAACTAGCTGCCTTCA1250.3697240379780532No Hit
GAATAAAAGAGAGCAAGATTAGACTTTATATTTCAGAGCCTTGGTCTAAC1120.3312727380283357No Hit
TCCCAATACAGTATTTCTTTCTTAAAAATAAATTCAACAAGTTTGCTGAA1020.3016948149900914No Hit
GTCTTGGAGAGAGTGTAGTTATAACCATCCTTTCCAGCATACTTCTGGAA830.24549676121742733No Hit
GAGTACATGGGGGGGCGTGGGTTGAGGAGAGGCTCCAGACCCGCACGCCG810.23958117660977846No Hit
GTCAAGGACACCAGGGTCCTTCTGGTTCTTTGTGAAGGCAGCTAGTTCTG800.23662338430595403No Hit
GTATCAACGCAGAGTACATGGGGGGGCGTGGGTTGAGGAGAGGCTCCAGA780.23070779969830518No Hit
GCCCTACAGAGACTGAGCGGTGCATCGAGTCCCTGATTGCTGTCTTCCAG730.21591883817918306No Hit
GCTCCAACATGGCAAAAATCTCCAGCCCTACAGAGACTGAGCGGTGCATC710.21000325357153424No Hit
GCCATAGCTAGGCCACCAATCAGATTAAGAAATTCTGAGAAATCTAGCTG700.20704546126770976No Hit
GTACATGGGATTACCAGAGGAAAAATAAACATTTCTCAGTAACTTTGCCT690.20408766896388536No Hit
GCCTTGAGGAAGGAGTCATGGCAAGCCATAGCTAGGCCACCAATCAGATT690.20408766896388536No Hit
GTCTTTATACTGCAAACTATTTAAAGAAATATGTATTCTGTAAAAGAAAA690.20408766896388536No Hit
GTATTAATTTGTAAGAATATGCATCTTAAAATGGCAAGTTTTCCATATTT660.19521429205241206No Hit
GTTATAACCATCCTTTCCAGCATACTTCTGGAAGACAGCAATCAGGGACT660.19521429205241206No Hit
CCTCAATATTTTACTTTGAGAGGACATTAATTTCCATTTCAAAATAAAAT650.19225649974858766No Hit
GTACATGGGAAAGTTTAATTGAAAGAATGTAAAGCAACCAAAAAGAAAAA640.18929870744476324No Hit
GAATATGCATCTTAAAATGGCAAGTTTTCCATATTTTTACAACTCACTGG630.18634091514093878No Hit
GTCCTTGACCGCATGATGAAGAAACTGGACACCAACAGTGATGGTCAGCT630.18634091514093878No Hit
GCCTTAAAGATACAGTACCCCTCTAGCTTTGTTTAGTCTTTATACTGCAA600.1774675382294655No Hit
CTCCAGACCCGCACGCCGCGCGCACAGAGCTCTCAGCGCCGCTCCCAGCC600.1774675382294655No Hit
CATTTAAGGTGTAGTATTAATAGGTTAAAACCAGGCTTTCTAGAAAGAAT600.1774675382294655No Hit
ATACTACACCTTAAATGTGTCCAAAAACAGTTCTGAGTGAGACTGTAATT600.1774675382294655No Hit
GGATCACACTCATTGATGAAGAAAACTGTAACAGCACAGAATAAAAGAGA590.1745097459256411No Hit
CTTTTATTCTGTGCTGTTACAGTTTTCTTCATCAATGAGTGTGATCCAGT580.1715519536218167No Hit
ATACAGAACTAGCTGCCTTCACAAAGAACCAGAAGGACCCTGGTGTCCTT580.1715519536218167No Hit
TTGTACACCCATGAAAGAAAACTTTTATGCAAGGTCTTGCATTTAAAAGA570.16859416131799224No Hit
ATGCAAGACCTTGCATAAAAGTTTTCTTTCATGGGTGTACAAAGAATGCG570.16859416131799224No Hit
GTACATGGGAAGACAGTGTTTGACGAAGCGATCCGAGCAGTCCTCTGCCC560.16563636901416784No Hit
ATTAATACTACACCTTAAATGTGTCCAAAAACAGTTCTGAGTGAGACTGT560.16563636901416784No Hit
TAACTACACTCTCTCCAAGACAGAGTTCCTAAGCTTCATGAATACAGAAC550.1626785767103434No Hit
ACATGGGGGGGCGTGGGTTGAGGAGAGGCTCCAGACCCGCACGCCGCGCG550.1626785767103434No Hit
GTCTTGCATTTAAAAGACAGCTTTGCGAATATTTTGTAAATTACAGTCTC540.15972078440651896No Hit
GTATCAACGCAGAGTACATGGGAAAGTTTAATTGAAAGAATGTAAAGCAA530.15676299210269456No Hit
GTATAAAGACTAAACAAAGCTAGAGGGGTACTGTATCTTTAAGGCAGTAC520.1538051997988701No Hit
AGTTTATTCTTTCTAGAAAGCCTGGTTTTAACCTATTAATACTACACCTT510.1508474074950457No Hit
GGTTATAACTACACTCTCTCCAAGACAGAGTTCCTAAGCTTCATGAATAC490.14493182288739684No Hit
CTGTAGGGCTGGAGATTTTTGCCATGTTGGAGCTGAGCGAGGCGCGGGAG480.1419740305835724No Hit
CTTTATAACATTGCTTCTTGTTAAAACATTGTTATCAAGTAACAGTAACT480.1419740305835724No Hit
CTTCTGGAAGACAGCAATCAGGGACTCGATGCACCGCTCAGTCTCTGTAG480.1419740305835724No Hit
GCTTTATTACTATTGGCAGGTGGGGCCTGCATGAGGTGGTTAGTGTGCTC470.13901623827974802No Hit
GCATTAATACGCCCATTATGGTCATTTAAATTGGGGTTTATTTCAGCAAA460.13605844597592356No Hit
TTCCAGAAGTATGCTGGAAAGGATGGTTATAACTACACTCTCTCCAAGAC460.13605844597592356No Hit
CTACAGAGACTGAGCGGTGCATCGAGTCCCTGATTGCTGTCTTCCAGAAG450.13310065367209914No Hit
TATTAATGCTATGGTTATCAATCTCCAAGAATATTGCTATTTTCATGTCC440.13014286136827474No Hit
ACTACACTCTCTCCAAGACAGAGTTCCTAAGCTTCATGAATACAGAACTA440.13014286136827474No Hit
GTCTAACTCCCTTCAGTTTCGGTTGGTATCAGTTATGTTTTCTAACCAGA430.1271850690644503No Hit
GACCATCACTGTTGGTGTCCAGTTTCTTCATCATGCGGTCAAGGACACCA430.1271850690644503No Hit
GTGCATCGAGTCCCTGATTGCTGTCTTCCAGAAGTATGCTGGAAAGGATG430.1271850690644503No Hit
GAGTGAGACTGTAATTTACAAAATATTCGCAAAGCTGTCTTTTAAATGCA420.12422727676062587No Hit
TCACTGTTGGTGTCCAGTTTCTTCATCATGCGGTCAAGGACACCAGGGTC420.12422727676062587No Hit
TGGGAAGACAGTGTTTGACGAAGCGATCCGAGCAGTCCTCTGCCCGCCTC420.12422727676062587No Hit
CATTGATGAAGAAAACTGTAACAGCACAGAATAAAAGAGAGCAAGATTAG410.12126948445680145No Hit
GTATCAACGCAGAGTACATGGGAAGACAGTGTTTGACGAAGCGATCCGAG410.12126948445680145No Hit
GTATCAACGCAGAGTACATGGGATTACCAGAGGAAAAATAAACATTTCTC410.12126948445680145No Hit
CTATTAATACTACACCTTAAATGTGTCCAAAAACAGTTCTGAGTGAGACT410.12126948445680145No Hit
GTACAAAGGTTCCAAGGGACAGGACCAAGAACGAGGGGCTGAGACATTTA400.11831169215297702No Hit
GTATTCATGAAGCTTAGGAACTCTGTCTTGGAGAGAGTGTAGTTATAACC400.11831169215297702No Hit
GTTCTGAGTGAGACTGTAATTTACAAAATATTCGCAAAGCTGTCTTTTAA380.11239610754532815No Hit
GTCTCTGTAGGGCTGGAGATTTTTGCCATGTTGGAGCTGAGCGAGGCGCG380.11239610754532815No Hit
ATAAAATATCTTATGAAAAACTGGATCACACTCATTGATGAAGAAAACTG380.11239610754532815No Hit
GAACTAGCTGCCTTCACAAAGAACCAGAAGGACCCTGGTGTCCTTGACCG370.10943831524150374No Hit
GGTTAGAAAACATAACTGATACCAACCGAAACTGAAGGGAGTTAGACCAA370.10943831524150374No Hit
CATTGGTACTGCCTTAAAGATACAGTACCCCTCTAGCTTTGTTTAGTCTT370.10943831524150374No Hit
AGCTATGGCTTGCCATGACTCCTTCCTCAAGGCTGTCCCTTCCCAGAAGC360.10648052293767932No Hit
GAACCAGAAGGACCCTGGTGTCCTTGACCGCATGATGAAGAAACTGGACA360.10648052293767932No Hit
CATTATGGTCATTTAAATTGGGGTTTATTTCAGCAAACTTGTTGAATTTA350.10352273063385488No Hit
GAAAGGGGGTGGGTTTGAAGGCCAGGGCCAAGGGGTCCTCAGGTCCGCTT350.10352273063385488No Hit
GTATTAATAGGTTAAAACCAGGCTTTCTAGAAAGAATAAACTTACATATT350.10352273063385488No Hit
GGTATCAACGCAGAGTACATGGGGGGGCGTGGGTTGAGGAGAGGCTCCAG350.10352273063385488No Hit
GGTATCAACGCAGAGTACATGGGAAAGTTTAATTGAAAGAATGTAAAGCA350.10352273063385488No Hit
GTTGAGGAGAGGCTCCAGACCCGCACGCCGCGCGCACAGAGCTCTCAGCG350.10352273063385488No Hit
TCTTAAAATGGCAAGTTTTCCATATTTTTACAACTCACTGGTGGTTTTCC350.10352273063385488No Hit
GTCTAATCTTGCTCTCTTTTATTCTGTGCTGTTACAGTTTTCTTCATCAA350.10352273063385488No Hit
GCCTAATGATGCATCAAATACAAAAACATATAATACATCAATAGTCAACC350.10352273063385488No Hit
CCCATGAAAGAAAACTTTTATGCAAGGTCTTGCATTTAAAAGACAGCTTT350.10352273063385488No Hit
CTTCTGGGAAGGGACAGCCTTGAGGAAGGAGTCATGGCAAGCCATAGCTA350.10352273063385488No Hit
GGTATCAACGCAGAGTACATGGGATTACCAGAGGAAAAATAAACATTTCT350.10352273063385488No Hit
TTCTTTGTACACCCATGAAAGAAAACTTTTATGCAAGGTCTTGCATTTAA340.10056493833003047No Hit
CACTGGTGGTTTTCCGCATTCTTTGTACACCCATGAAAGAAAACTTTTAT340.10056493833003047No Hit
GGCGTGGGTTGAGGAGAGGCTCCAGACCCGCACGCCGCGCGCACAGAGCT340.10056493833003047No Hit
AGATTAGACTTTATATTTCAGAGCCTTGGTCTAACTCCCTTCAGTTTCGG340.10056493833003047No Hit
GGGTTGAGGAGAGGCTCCAGACCCGCACGCCGCGCGCACAGAGCTCTCAG340.10056493833003047No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)