FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001421870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001421870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20808
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTACATGACATGTTTTTCTTAAAAACAATGCCTCCACTCCAAATAAAT1440.6920415224913495No Hit
GTTTAGAAATGGAAAAATGTTAACAAATGTGGCAATTATTTTGGATCTAT980.47097270280661285No Hit
ACCGTGAGGATGGTGTGACCCCATATATGATTTTCTTTAAGGATGGTTTA710.34121491733948484No Hit
GTATTGGACTACCGTGAGGATGGTGTGACCCCATATATGATTTTCTTTAA670.3219915417147251No Hit
GTACATGGGTATTGGACTACCGTGAGGATGGTGTGACCCCATATATGATT660.31718569780853517No Hit
GTGTGACCCCATATATGATTTTCTTTAAGGATGGTTTAGAAATGGAAAAA600.28835063437139563No Hit
GTCTTAAGTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATG540.25951557093425603No Hit
ATATATGATTTTCTTTAAGGATGGTTTAGAAATGGAAAAATGTTAACAAA530.25470972702806616No Hit
TAAATGAAGAGCTCAAGATGACATCAGTCCCATTTGTCTTAAGTCCTGGT530.25470972702806616No Hit
CTTAAAAACAATGCCTCCACTCCAAATAAATCACAGTCAAAATAAATGAA500.24029219530949633No Hit
ATTTTGGATCTATCACCTGTCATCATAACTGGCTTCTGCTTGTCATCCAC500.24029219530949633No Hit
GCTCAAGATGACATCAGTCCCATTTGTCTTAAGTCCTGGTGTTGTGTGGA470.22587466359092656No Hit
GTATCAACGCAGAGTACATGGGTATTGGACTACCGTGAGGATGGTGTGAC410.19703960015378702No Hit
GTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTG400.1922337562475971No Hit
AAATAAATCACAGTCAAAATAAATGAAGAGCTCAAGATGACATCAGTCCC400.1922337562475971No Hit
CCTCCACTCCAAATAAATCACAGTCAAAATAAATGAAGAGCTCAAGATGA390.18742791234140715No Hit
CTACATGACATGTTTTTCTTAAAAACAATGCCTCCACTCCAAATAAATCA390.18742791234140715No Hit
CATCATAACTGGCTTCTGCTTGTCATCCACACAACACCAGGACTTAAGAC380.18262206843521722No Hit
CATCAGTCCCATTTGTCTTAAGTCCTGGTGTTGTGTGGATGACAAGCAGA360.17301038062283738No Hit
CTTTAAGGATGGTTTAGAAATGGAAAAATGTTAACAAATGTGGCAATTAT330.15859284890426759No Hit
AAATAAATGAAGAGCTCAAGATGACATCAGTCCCATTTGTCTTAAGTCCT330.15859284890426759No Hit
TCTATCACCTGTCATCATAACTGGCTTCTGCTTGTCATCCACACAACACC330.15859284890426759No Hit
TATCAACGCAGAGTACATGGGTATTGGACTACCGTGAGGATGGTGTGACC310.14898116109188772No Hit
GTTTAAATGCATTTTATTTTTAGACAACCTACATGACATGTTTTTCTTAA290.13936947327950788No Hit
TTTTTAGACAACCTACATGACATGTTTTTCTTAAAAACAATGCCTCCACT280.13456362937331795No Hit
GAGTACATGGGTATTGGACTACCGTGAGGATGGTGTGACCCCATATATGA270.12975778546712802No Hit
GGACTACCGTGAGGATGGTGTGACCCCATATATGATTTTCTTTAAGGATG260.1249519415609381No Hit
CAATGCCTCCACTCCAAATAAATCACAGTCAAAATAAATGAAGAGCTCAA250.12014609765474817No Hit
GGTATCAACGCAGAGTACATGGGTATTGGACTACCGTGAGGATGGTGTGA250.12014609765474817No Hit
ACGCAGAGTACATGGGTATTGGACTACCGTGAGGATGGTGTGACCCCATA250.12014609765474817No Hit
TTTAAATGCATTTTATTTTTAGACAACCTACATGACATGTTTTTCTTAAA250.12014609765474817No Hit
GTCCCATTTGTCTTAAGTCCTGGTGTTGTGTGGATGACAAGCAGAAGCCA240.11534025374855825No Hit
GTTATGATGACAGGTGATAGATCCAAAATAATTGCCACATTTGTTAACAT240.11534025374855825No Hit
AGATGACATCAGTCCCATTTGTCTTAAGTCCTGGTGTTGTGTGGATGACA240.11534025374855825No Hit
GTCAAAATAAATGAAGAGCTCAAGATGACATCAGTCCCATTTGTCTTAAG240.11534025374855825No Hit
CATATATGATTTTCTTTAAGGATGGTTTAGAAATGGAAAAATGTTAACAA240.11534025374855825No Hit
CCTGTCATCATAACTGGCTTCTGCTTGTCATCCACACAACACCAGGACTT210.10092272202998846No Hit
GGTTTAGAAATGGAAAAATGTTAACAAATGTGGCAATTATTTTGGATCTA210.10092272202998846No Hit
CCTACATGACATGTTTTTCTTAAAAACAATGCCTCCACTCCAAATAAATC210.10092272202998846No Hit
GTGTTGTGTGGATGACAAGCAGAAGCCAGTTATGATGACAGGTGATAGAT210.10092272202998846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)